Method for cultivating pearl by inducing triploid hyriopsis cumingii

A technology of spinnaker mussels and triploids, applied in fish farming, application, climate change adaptation, etc., can solve problems such as differences in reproductive biology, fertilization and embryo development between spinnaker mussels and marine shellfish, and achieve suitable promotion, Easy source, the effect of increasing the success rate of induction

Inactive Publication Date: 2012-03-21
ZHEJIANG SHANXIAHU PEARL GROUP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the unique mussel species in our country is also the main mussel species used for freshwater pearl culture to induce triploids of Trichophyllus sinensis. There are few related reports, only CN1957686A, which adopts the type of in vitro fertilization and embryonic development, and Its in vitro fertilization uses the eggs produced by the triploid S. sinensis and the sperm of the diploid S. sinensis, which itself needs the triploid S. sinensis as a parent.
[0006] Although the triangular sail clam and other marine molluscs are at the same level in biological classification and evolution, and have many similarities, there are many differences in reproductive biology between the triangular sail clam and marine molluscs, especially in the aspects of fertilization and embryonic development. big difference

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] 1 Selection and sex identification of parent mussels

[0046] To select 3-year-old triangular sail mussels, the shell length should be more than 15cm, the shell thickness should be more than 5cm, the shell should be intact, the mussel body should be strong, the abdomen should be round, the adductor muscle should be strong and powerful, and there should be no disease or injury. Then the male and female should be determined. Parent mussels are selected according to female: male = 1:1.

[0047] 2 Cultivation of broodstock

[0048] From November to February of the following year, separate the male and female mussels from the parent mussels and hang them in the micro-flow water of the pond. The water depth is about 1.5m. The water quality is required to be fresh, pollution-free, fat and thin, and the transparency is about 30cm. , about 600 per mu are suspended and raised. During the breeding season (early March), the female and male mussels are cultivated separately and ...

Embodiment 2

[0134] 1 Selection and sex identification of parent mussels

[0135] To select 4-year-old triangular sail mussels, it is required that the shell length is more than 15cm, the shell thickness is more than 5cm, the shell is intact, the mussel body is strong, the abdomen is round, the adductor muscle is strong and powerful, and there is no disease or injury, and then the sex is determined. Parent mussels are selected according to female: male = 2:1.

[0136] 2 Cultivation of broodstock

[0137] From November to February of the following year, separate the male and female mussels from the parent mussels and hang them in the micro-flow water of the pond. The water depth is about 1.5m. The water quality is required to be fresh, pollution-free, fat and thin, and the transparency is about 30cm. , about 600 per mu are suspended and raised. During the breeding season (early March), the female and male mussels are cultivated separately and strictly isolated.

[0138] 3 triploid indu...

Embodiment 3

[0165] 1 Selection and sex identification of parent mussels

[0166] To select a 6-year-old triangular sail clam, it is required that the shell length is more than 15cm, the shell thickness is more than 5cm, the shell is intact, the mussel body is strong, the abdomen is round, the adductor muscle is strong, and there is no disease or injury, and then the sex is determined. Parent mussels were selected according to female: male = 3:1.

[0167] 2 Cultivation of broodstock

[0168] From November to February of the following year, separate the male and female mussels from the parent mussels and hang them in the micro-flow water of the pond. The water depth is about 1.5m. The water quality is required to be fresh, pollution-free, fat and thin, and the transparency is about 30cm. , about 600 per mu are suspended and raised. During the breeding season (early March), the female and male mussels are cultivated separately and strictly isolated.

[0169] 3 triploid induction

[017...

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PUM

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Abstract

The invention discloses a method for cultivating a pearl by inducing triploid hyriopsis cumingii. The method comprises the following steps of: cultivating the triploid hyriopsis cumingii by induction, namely selecting diploid hyriopsis cumingii with age of 3 to 6, shell length of over 15cm and shell thickness of over 5cm in normal growth as parent clams; isolating the male clams from the female clams of the selected parent clams for cultivation respectively; tracking and observing the development condition of the eggs of the female clams; transferring the female clams into a pond for cultivating the male clams to perform natural insemination on the female clams after the eggs are maturely developed; observing the development condition of the fertilized eggs; putting the female clams into 300 to 450 mu mol / L aqueous solution of 6-dimethyl aminopurine after over 80 percent of the fertilized eggs have first polar bodies; and performing induction processing for 10 to 30 minutes to obtain induced female clams; cultivating the female clams, and hatching and raising the seedlings to obtain the triploid juvenile clams; and performing cultivation to obtain the triploid hyriopsis cumingii. In the method, the normal diploid hyriopsis cumingii is used as the parents, and the natural insemination is adopted, so that the induction success rate is improved to over 70 percent.

Description

technical field [0001] The invention belongs to the technical field of pearl cultivation, and in particular relates to a method for inducing and cultivating triploid S. sinensis and using the cultivated triploid S. sinensis to cultivate freshwater pearls. Background technique [0002] Freshwater pearl cultivation has many years of history and cultivation experience in my country and has formed a large scale, and plays an important role in freshwater cultivation. However, with the development of the pearl culture industry and the continuous maturation of artificial propagation techniques, the degradation of pearl mussel germplasm resources and the occasional occurrence of diseases caused by long-term multi-generation inbreeding have brought certain harm to the culture industry. The degeneration of the disease is concentrated in premature sexual maturity, and the resulting slow growth and low disease resistance. The main problems faced by pearl culture are the long breeding p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 弭忠祥陈海军韩继卫杨受保刘小锋刘文东
Owner ZHEJIANG SHANXIAHU PEARL GROUP CO LTD
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