Method for cold preservation of mytilus coruscus D-shape larvas
A technology of thick-shelled mussels and a preservation method, which is applied in fish farming, application, climate change adaptation, etc., can solve problems such as insufficient large-scale artificial propagation technology of thick-shelled mussels, short supply of seedlings, and unstable number of seedlings.
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Embodiment 1
[0027] The method for refrigerated storage of D-shaped larvae of thick-shelled mussels is characterized in that it comprises the following steps:
[0028] (1) Screening of D-shaped larvae:
[0029] Newly hatched mussel D-shaped larvae (also called D-shaped larvae) are screened, and the D-shaped larvae of the thick-shelled mussel are D-shaped larvae with regular shape.
[0030] (2) Cold storage of D-shaped larvae:
[0031] Select the newly hatched D-shaped larvae of the thick-shelled mussel, put them into a 2L beaker filled with seawater at 18°C, and the density of the larvae in the beaker is 5 / ml; then, put the beaker into a refrigerator at 4°C for preservation. And throw bait.
[0032] During refrigerated storage, replace seawater at 3-5°C every four days. The low-temperature seawater is filtered through a mixed fiber membrane with a salinity of 30‰; use a 40μm filter when replacing low-temperature seawater.
[0033] Feed Chaetoceros gracilis once every two days, and the f...
Embodiment 2
[0040] The method for refrigerated storage of D-shaped larvae of thick-shelled mussels is characterized in that it comprises the following steps:
[0041] (1) Screening of D-shaped larvae:
[0042] Newly hatched mussel D-shaped larvae (also called D-shaped larvae) are screened, and the D-shaped larvae of the thick-shelled mussel are D-shaped larvae with regular shape.
[0043] (2) Cold storage of D-shaped larvae:
[0044] Select the newly hatched D-shaped larvae of the thick-shelled mussel and put them into a 2L beaker filled with seawater at 17°C. The density of the larvae in the beaker is 5 / ml; And throw bait.
[0045] During refrigerated storage, replace the 5°C seawater every four days. The low-temperature seawater is filtered through the mixed fiber membrane, and the salinity is 30‰; when replacing the low-temperature seawater, use a 40μm filter.
[0046] Feed golden algae once every two days, and the feeding density is 5×10 4 cells / ml.
[0047] Furthermore, the surv...
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