Steroid pretreatment and detection method

Abstract

The invention relates to a steroid pretreatment and detection method. The method comprises the following steps: (1) sampling; (2) mixing a sample with extractant evenly to treat; (3) mixing the obtained supernatant and distilled water; (4) placing the mixed liquid in a special solid phase extraction column to perform solid phase extraction; (5) eluting the solid phase extraction column with abluent; (6) eluting the solid phase extraction column with eluant to obtain components under test; (7) obtaining eluent to treat with the microporous filter membrane; and (8) analyzing and determining the steroid components. By adopting the method of the invention, eleven steroid substances such as testosterone, methyltestosterone and methandrostenolone can be detected at once, the problem that multi-residue analysis can not be performed is solved; the novel extractant and special steroid solid phase extraction column are adopted, the extraction efficiency is high, the purification effect is good, the problems of low extraction efficiency and poor purification effect are solved; and the detection speed and accuracy are high, and better technical effect can be obtained.

Description

technical field [0001] The invention relates to a pretreatment method of steroids, which belongs to the field of food safety. Background technique [0002] The production-promoting effect of steroid drugs is multifaceted, mainly manifested as promoting protein synthesis, increasing basal metabolism, improving feed conversion rate, reducing material consumption, etc., which can enhance the deposition of substances in the body and improve production performance, and can quickly produce significant and direct effects. Economical and therefore very attractive to producers. The use of hormones (non-therapeutic purposes) in animal husbandry has a history of nearly 50 years. There are quite a few feed manufacturers, feed distributors and professional breeding households. For example, diethylstilbestrol is used in poultry farming. Add it to feed livestock and poultry or bury it under the ears and wings of poultry to promote animal growth and increase the lean meat ratio of livestoc...

AI Technical Summary

Problems solved by technology

The pretreatment process requires multiple extractions, centrifugation, liquid-liquid extraction, and rotary evaporation. The process is cumbersome, difficult to master, and requires high operating requirements for experimenters; some test items require enzymatic hydrolysis, and most of them require more than 15 hours of enzymatic The solution process makes the detection time very long; for a wide variety of steroids, most methods can only detect a single item, and cannot achieve multi-residue analysis, which greatly increases the detection cost; in addition, there are low extraction efficiency and poor purification effect. question

Images