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Cell preserving fluid for liquid-based cytologic diagnosis

A technology of cytology and preservation solution, which is applied in the preservation of human or animal bodies, the preparation and application of test samples, etc., and can solve the problems of cells that cannot be preserved for a long time, cell deformation, cell degeneration, etc.

Inactive Publication Date: 2011-01-26
南京卡博生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, 40-50% alcohol is commonly used as a preservation solution. Although it can immobilize highly mobile proteins, the protein will immediately form deposits and cause cell deformation.
There are also alcohols whose content is less than 20%, but the cells cannot be preserved for a long time, and the cells are easy to degenerate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0007] The basic composition of a cell preservation solution for liquid-based cytology diagnosis of the embodiment includes: dextran, 4-hydroxyethylpiperazineethanesulfonic acid, ethanol, formaldehyde, potassium dihydrogen phosphate and ethylenediaminetetraacetic acid di sodium. The dextran of the present embodiment selects dextran T4, wherein the content of dextran T4 is 12g / L, the content of 4-hydroxyethylpiperazineethanesulfonic acid is 30g / L, the content of ethanol is 150g / L, The content of formaldehyde is 50g / L, the content of potassium dihydrogen phosphate is 20g / L, and the content of disodium edetate is 8g / L.

[0008] The content of 12g / L dextran in the cell preservation solution is an anti-drying agent, which can keep the cells moist during the production process, so that the shape does not change and the diagnostic value is maintained. 4-hydroxyethylpiperazineethanesulfonic acid with a content of 30 g / L and potassium dihydrogen phosphate with a content of 20 g / L are ...

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PUM

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Abstract

The invention relates to cell preserving fluid for liquid-based cytologic diagnosis. The cell preserving fluid comprises glucan, 4-hydroxyethyl piperazine ethanesulfonic acid, ethanol, formaldehyde, potassium dihydrogen phosphate and disodium ethylene diaminetetraacetate, wherein the glucan is at least one of glucan T4, glucan T6 and glucan T9. The problems that the cell shape is easy to change and the cell preserving fluid cannot be preserved for long time in the process of preparing sheets at present are solved.

Description

technical field [0001] The invention belongs to the field of cell preservation solution, in particular to a cell preservation solution for liquid-based cytological diagnosis. Background technique [0002] The cell preservation solution for liquid-based cytology diagnosis refers to a solution used to preserve cells collected from the human body to perform liquid-based cytology detection of cells for cytopathological diagnosis. Liquid-based cytology tests are used to test gynecological samples, such as cervical cells, and non-gynecological samples, such as saliva, urine, etc. In liquid-based cytology, the collected cells are examined for the presence of pathological cells by using a microscope. In order to perform the above liquid-based cytology assay, a cell preservation solution for preserving the collected cells is required. [0003] The existing preservation solution is easy to change the shape of the cells during the production process, and loses the diagnostic value. ...

Claims

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Application Information

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IPC IPC(8): A01N1/00G01N1/28
Inventor 吕军赵世鹏
Owner 南京卡博生物科技有限公司