Composition comprising the extracts of lysimachia clethroides for prevention and treatment of cardiovascular diseases

A technology for preventing and treating cardiovascular disease and a composition, which is applied in the field of functional health food to achieve the effect of strong vasodilation effect

Inactive Publication Date: 2011-02-09
YANG JI CHEM CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the article discloses that despite the high concentration of the crude extract, up to 4000 μg / ml, the extract has only a slight inhibitory activity against angiotensin converting enzyme

Method used

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  • Composition comprising the extracts of lysimachia clethroides for prevention and treatment of cardiovascular diseases
  • Composition comprising the extracts of lysimachia clethroides for prevention and treatment of cardiovascular diseases
  • Composition comprising the extracts of lysimachia clethroides for prevention and treatment of cardiovascular diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment 1: the preparation of the crude extract of pearl vegetable

[0065]Pearl vegetables collected from the province of Kangwon-do are rinsed with water to remove impurities. Then dry and pulverize. In the extraction container, add 25g of pulverized pearls, and a total amount of 500ml70wt% ethanol aqueous solution, and carry out heat extraction at 70°C under reflux cooling, repeat three times, each for three hours. The resulting product was filtered with filter paper, and the filtrate was concentrated under reduced pressure in a 40°C water bath and lyophilized. As a result, 5.3 g of a crude extract of loosestrife was obtained.

Embodiment 2

[0066] Embodiment 2: the preparation of the fraction of loose extract of pearl

[0067] The crude extract of loosestrife obtained in Example 1, each part of 5g, was suspended in 50ml of purified water respectively, in the order of hexane, ethyl acetate and n-butanol, each solvent 50ml, and the obtained product was subjected to sequential solvent fractionation respectively , extracted three times to obtain fractions of each solvent respectively, and concentrated these fractions under reduced pressure. As a result, the hexane fraction, ethyl acetate fraction and n-butanol fraction of loosestrife were obtained.

experiment example 1

[0068] Experimental Example 1: Determination of the Inhibitory Effect of the Crude Extract of Pearl Vegetables on NAD(P)H Oxidase Activity

[0069] The effect of the crude extract and fractions obtained in the above-mentioned Examples 1 and 2 on inhibiting the activity of NAD(P)H oxidase was determined, and NAD(P)H oxidase is an index enzyme for the development of cardiovascular diseases. For this purpose, rat arterial smooth muscle cells (rat arterial smooth muscle cells; RASMC) and bovine vascular endothelial cells (bovine aortic endothelial cells; BAECs) were used to compare the changes in NAD(P)H oxidase activity. See Table 1 for the results. First, arterial smooth muscle cells and vascular endothelial cells were mixed with MEM (minimal essential medium), DMEM (Dulbecco's minimal medium) and 10% FBS (fetal bovine serum) solutions, respectively, and these cells were incubated in 5% CO 2 / 37°C and cultured in 96-well plates for 24 hours. They were then cultured for anoth...

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Abstract

The present invention relates to a composition for treatment and prevention of cardiovascular diseases, containing an extract of Lysimachia clethroides. More particularly, the extract of Lysimachia clethroides strongly inhibits NAD(P)H oxidases, which are the main cause of vascular diseases, and at the same time, regulates contraction and relaxation of vascular smooth muscles to manifest a potent vasorelaxant effect, thereby improving the blood pressure regulation and vascular endothelial dysfunction. Therefore, a composition containing this as an active ingredient can be usefully utilized as a pharmaceutical prodvct or health functional food prodvct for the prevention and treatment of cardiovascular diseases.

Description

technical field [0001] The invention relates to a composition containing the extract of Lindera obtusiloba for preventing and treating cardiovascular diseases, and functional health food. More specifically, the present invention is characterized by containing, as an active ingredient, an extract of nacelle, which inhibits oxidative stress by directly inhibiting NAD(P)H oxidase, while activating endothelial nitric oxide synthase, thereby modulating The contraction and relaxation of vascular smooth muscle cells have the effect of preventing and treating cardiovascular diseases. Background technique [0002] According to the World Health Organization (WHO) report, in 1999, the mortality rate of cardiovascular disease worldwide was 30% or higher, and by 2010, cardiovascular disease will become the number one cause of death in developed countries. Diseases, and in Asian countries including Japan, South Korea, etc., the growth rate of cardiovascular diseases is particularly high....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185
CPCA61K36/185A61P9/02A46B17/065A47K1/09A47K5/18A61L2/10
Inventor 玉珉镐李廷钰崔云善孙正德金钟勋林智雄李正根
Owner YANG JI CHEM CO LTD
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