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Sers-based, single step, real-time detection of protein kinase and/or phosphatase activity

A phosphatase activity, phosphatase technology, applied in the field of kinase and/or phosphatase detection systems

Inactive Publication Date: 2011-02-09
RGT UNIV OF CALIFORNIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods are limited by reagent availability, well coating, and multiple washing and incubation steps

Method used

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  • Sers-based, single step, real-time detection of protein kinase and/or phosphatase activity
  • Sers-based, single step, real-time detection of protein kinase and/or phosphatase activity
  • Sers-based, single step, real-time detection of protein kinase and/or phosphatase activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0125] Fabrication and use of SERs microarray

[0126] Fabrication of Nanocone SERS Substrates

[0127] Starting from a monocrystalline silicon wafer, a thin 300 nm thick layer of polycrystalline silicon is deposited on the polished top surface of the silicon wafer. Microscale devices can be patterned on the polysilicon surface using photolithography (photolithography). After patterning, the silicon wafer was etched in a plasma assisted reactive ion etcher. The etching methods for preparing nanopyramidal SERS substrates are different from those used in conventional silicon film etching. First, by using SF 6 Plasma etching for 10 seconds strips the native oxide layer on the polysilicon film. Next, make O 2 and HBr gas were flowed in the RF plasma etch chamber for 7 seconds to define nanoscale oxide islands on top of the polysilicon film. These nanoscale oxide islands are created by simultaneous etching and oxidation processes. The average diameter of the oxide islands is...

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Abstract

This invention provides novel compositions and methods for the detection, and / or quantification, of the presence and / or activity of one or more kinases and / or phosphatases. In certain embodiments this invention a device for the detection of kinase and / or phosphatase activity where the device comprises a Raman active surface comprising features that enhance Raman scattering having attached thereto a plurality of kinase and / or phosphatase substrate molecules.

Description

[0001] Cross References to Related Applications [0002] This application claims priority and benefit to USSN 61 / 018,286, filed December 31, 2007, and USSN 61 / 022,115, filed January 18, 2008, both of which are hereby incorporated by reference in their entirety. [0003] Statement of Rights to Inventions Made Under Federally Supported Research and Development [0004] This work was supported in part by Grant No: DE-AC02-05CH11231 from the US Department of Energy. The US Government has certain rights in this application. technical field [0005] The present invention relates to the field of diagnostic and screening devices. Specifically, in some embodiments, the present invention provides kinase and / or phosphatase detection systems comprising one or more kinases and / or phosphatases attached to a surface comprising nanoscale features that enhance Raman spectroscopic signal substrate. Background technique [0006] The addition or removal of phosphate groups (phosphate groups...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01J3/44C12Q1/42C12Q1/48G01N33/50
CPCB01J2219/00677B01J2219/00382C12Q1/42B01J2219/00635B01J2219/00612C40B50/18G01N2500/04B01J2219/00585B01J2219/00702G01N21/658B01J2219/00596B01J2219/00605B01J2219/00527B82Y30/00C40B60/12B01J2219/00725C12Q1/485B81C1/00206B01J2219/00576B01J2219/00621B01J2219/00648B01J2219/00659B81B2201/0214B01J2219/0063B01J2219/00637B01J2219/0074B01J2219/00626B01J19/0046B01J2219/00734
Inventor 陈帆青刘刚乔纳森·A·埃尔曼
Owner RGT UNIV OF CALIFORNIA
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