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Propagation method for directly inducing bulblet from lilium longiflorum aseptic seedling leaf

A technology for small bulbs and sterile seedlings, which is applied in the field of plant tissue culture, can solve the problems of degeneration of seed characteristics, easy death of test-tube seedlings, etc., and achieves the effect of uniform size.

Active Publication Date: 2013-01-16
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although there are many reports on lily scale cuttings and tissue rapid propagation, scale cuttings are likely to cause the problem of species degradation, and tissue rapid propagation is rarely used in reproduction, because a large number of test-tube seedlings are prone to die when transplanted
Since these methods are still unable to meet the production needs of lily bulbs, it is necessary to explore new methods for rapid propagation of lily bulbs and form a related technical system for bulb production

Method used

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  • Propagation method for directly inducing bulblet from lilium longiflorum aseptic seedling leaf
  • Propagation method for directly inducing bulblet from lilium longiflorum aseptic seedling leaf
  • Propagation method for directly inducing bulblet from lilium longiflorum aseptic seedling leaf

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Induction of lily scales: select 'Siberia' lily scales purchased from Beijing Greener Co., Ltd. without pests and diseases, wash them with detergent, rinse them under tap water for 30 minutes, soak them in 75% alcohol for 35 seconds, rinse them twice with sterile water, and then Sterilize with 0.1% mercuric chloride for different times, that is, the outer scales are sterilized for 15 minutes, the middle scales are sterilized for 12 minutes, and the inner scales are sterilized for 10 minutes, rinsed with sterile water for 4 to 5 times, and then cut in a culture dish with sterile filter paper. block spare.

[0021] Inoculate the above surface sterilized lily scales to MS+6-BA2.0mg / L+NAA0.5mg / L; MS+6-BA2.0mg / L+NAA 0.1mg / L; MS+6-BA2.0mg / L L+2,4-D 0.5mg / L; MS+6-BA2.0mg / L+2,4-D 0.1mg / L; MS+6-BA2.0mg / L+IAA0.1mg / L. Wherein, in the above medium, the sucrose content is 30g / L, and the agar content is 6.25g / L.

[0022] The results showed that the highest rate of adventitious bud ...

Embodiment 2

[0026] The clustered buds differentiated on the scales were cut into single buds and inoculated into the following eight media:

[0027] A: MS+6-BA1.0mg / L+NAA0.1mg / L;

[0028] B: MS+6-BA1.0mg / L+NAA0.5mg / L;

[0029] C: MS+6-BA1.5mg / L+NAA0.1mg / L;

[0030] D: MS+6-BA1.5mg / L+NAA0.5mg / L;

[0031] E: MS+6-BA2.0mg / L+NAA0.1mg / L;

[0032] F: MS+6-BA2.0mg / L+NAA0.5mg / L;

[0033] G: MS+6-BA2.5mg / L+NAA0.1mg / L;

[0034] H: MS+6-BA2.5mg / L+NAA0.5mg / L.

[0035] Wherein, in the above medium, the sucrose content is 30g / L, and the agar content is 6.25g / L.

[0036] The growth coefficient of clustered buds on A medium was 2.76, and the seedlings grew well; the growth coefficient of clustered shoots on B medium was 3.17, and the growth of seedlings was good; the growth coefficient of clustered shoots on C medium was 2.97, and the growth of seedlings was poor; The growth coefficient of clustered shoots on medium D was 3.96, and the seedlings were growing well; the growth coefficient of cluster...

Embodiment 3

[0039] Screening of culture medium for direct induction of bulblets from aseptic seedling leaves: Select the vigorously growing aseptic seedlings of ‘Siberia’, cut the leaves into leaf discs of about 3 cm, and inoculate them into the following four kinds of media, where the culture container is a petri dish:

[0040] MS+Pic (pyrazidine) 2.0mg / L+ sucrose 30g / L+ agar 6.25g / L;

[0041] MS+Pic (pyrazidine) 2.0mg / L+KT0.5mg / L+sucrose 30g / L+agar 6.25g / L;

[0042] MS+6-BA2.0mg / L+NAA 0.1mg / L+sucrose 30g / L+agar 6.25g / L;

[0043] MS+6-BA2.0mg / L+NAA 0.5mg / L+sucrose 30g / L+agar 6.25g / L.

[0044] Place the petri dish inoculated with leaf discs in a cardboard box, and then place the cardboard box in a completely dark artificial climate box. During the whole process, the culture temperature of the tissue culture seedlings is 20-23°C.

[0045]Among them, on the culture medium MS+Pic (pyrazidine) 2.0mg / L+sucrose 30g / L+agar 6.25g / L, the induction rate of single buds was 2.77%, while the cultur...

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Abstract

The invention provides a rapid propagation method for directly inducing a bulblet from a lilium longiflorum tissue culture seedling leaf. The method comprises the following steps of: inoculating a leaf disc of a lilium longiflorum aseptic seedling onto a bulblet inducing culture medium; and inoculating the induced bulblet onto a bulblet swelling culture medium, wherein the bulblet inducing culture medium consists of MS, 2.0 mg / L of 6-butyl acrylate (BA), 0.1 to 0.5 mg / L of naphthyl acetic acid (NAA), 30 g / L of cane sugar and 6.25 g / L of agar; and the bulblet swelling culture medium consists of MS, 2.0 to 2.5 g / L of active carbon, 90 g / L of cane sugar and 6.25 g / L of agar. In propagation method for directly inducing the bulblet from the lilium longiflorum aseptic seedling leaf, one leaf disc can produce 1 to 3 bulblets. The inductivity of a root directly produced by the bulblet is up to 100 percent. Compared with a process in which a cluster bud is directly induced from a scale and then the bulblet is produced, the method shortens time, simplifies steps and is a newly found direct organ generation measure.

Description

technical field [0001] The present invention relates to plant tissue culture, in particular to the tissue culture of Lilium longiflorum and the method for direct generation of leaf organs of sterile seedlings. Background technique [0002] In recent years, lily has been loved by people all over the world for its large flowers, rich colors and fragrant smell, and has quickly occupied the world's cut flower market. Among them, Asiatic lily hybrids, oriental lily hybrids, and musk lily hybrids are the main cut flower species. It is an indisputable fact that the bulbs used in cut flower production depend on imports. And imported lily bulbs tend to degenerate in the second year, so they need to be imported every year. [0003] Although there are many reports on the scale cutting and tissue rapid propagation of lily, the scale cutting is easy to cause the problem of species degradation, and the application of tissue rapid propagation is less, because a large number of test tube ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 张启翔石晋芳孙明程堂仁杨炜茹孔滢
Owner BEIJING FORESTRY UNIVERSITY