Method for efficiently enriching high-quality hydroxyl tyrosol from olive leaves

A technology for olive leaf hydroxytyrosol and hydroxytyrosol is applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., and can solve problems such as environmental pollution, general technicalities, poor selection of raw materials, etc. The effect of high rate and lower production cost

Active Publication Date: 2011-02-16
INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The Chinese patent No. 03815986 is a composition rich in hydroxytyrosol from olive plant liquid and its application method. It uses acid to hydrolyze oleuropein in olive leaves to obtain hydroxytyrosol, and then purifies it by chromatography. Some technologies use a large amount of Organic solvents, there are problems such as solvent loss and easy environmental pollution. The Chinese patent No. 200710057290 is a method for extracting hydroxytyrosol from olive extracts. The olive plant extracts are directly used for resin separation. At the same time, advanced The method of molecular distillation has promoted a technology for the development and utilization of olive leaf resources, but the technology is too general, and the raw materials are not well selected. Although some macroporous resins are used in the separation and purification process, they are not used. Comprehensive data parameters are given for comparison, so there are not small problems in production and application. How to design a set of economical production processes that can fit production applications and at the same time ensure comprehensive utilization of active ingredients in olive leaves has become a The essential

Method used

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  • Method for efficiently enriching high-quality hydroxyl tyrosol from olive leaves
  • Method for efficiently enriching high-quality hydroxyl tyrosol from olive leaves
  • Method for efficiently enriching high-quality hydroxyl tyrosol from olive leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] (1) the olive leaves are dried at low temperature and protected from light, and pulverized;

[0052] (2) Get the crushed olive leaves and use ultrasonic extraction, the ethanol consumption is 1-30 times, the alcohol concentration is 30-100%, the leaching temperature is 25-65 ℃, the leaching time is 10-30min, and the leaching times are three times;

[0053] (3) Filter the leachate, concentrate in vacuum, and the vacuum concentration temperature does not exceed 65° C. to obtain crude olive leaf extract. Dilute the crude extract with water and filter to remove impurities;

[0054] (4) In step (3), the crude product of the olive leaf alcohol extract obtained in further carries out ion exchange separation, and the method comprises the following steps:

[0055] a wet packed column, diameter = 2cm, height = 15cm, 30g AB-8;

[0056] b Dissolve the sample and add it to the chromatographic column of a;

[0057] c Add eluent, first wash with 2-3 times the volume of water, then...

Embodiment 2

[0063] (1) the olive leaves are dried at low temperature and protected from light, and pulverized;

[0064] (2) Get the crushed olive leaves and use ultrasonic extraction, the ethanol consumption is 1-30 times, the alcohol concentration is 30-100%, the leaching temperature is 25-65 ℃, the leaching time is 10-30min, and the leaching times are three times;

[0065] (3) Filter the leachate, concentrate in vacuum, and the vacuum concentration temperature does not exceed 65° C. to obtain crude olive leaf extract. Dilute the crude extract with water and filter to remove impurities;

[0066] (4) In step (3), the crude product of the olive leaf alcohol extract obtained in further carries out ion exchange separation, and the method comprises the following steps:

[0067] a Wet packed column, diameter = 2cm, height = 15cm, 30g DM130;

[0068] b Dissolve the sample and add it to the chromatographic column of a;

[0069] c Add eluent, first wash with 2-3 times the volume of water, the...

Embodiment 3

[0075] (1) the olive leaves are dried at low temperature and protected from light, and pulverized;

[0076] (2) Get the crushed olive leaves and use ultrasonic extraction, the ethanol consumption is 1-30 times, the alcohol concentration is 30-100%, the leaching temperature is 25-65 ℃, the leaching time is 10-30min, and the leaching times are three times;

[0077](3) Filter the leachate, concentrate in vacuum, and the vacuum concentration temperature does not exceed 65° C. to obtain crude olive leaf extract. Dilute the crude extract with water and filter to remove impurities;

[0078] (4) In step (3), the crude product of the olive leaf alcohol extract obtained in further carries out ion exchange separation, and the method comprises the following steps:

[0079] a Wet packed column, diameter = 2cm, height = 15cm, 30g D101;

[0080] b Dissolve the sample and add it to the chromatographic column of a;

[0081] c Add eluent, first wash with 2-3 times the volume of water, then ...

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Abstract

The invention discloses a method for efficiently enriching high-quality hydroxyl tyrosol from olive leaves. The method comprises the following steps of: sifting a raw production material with high content of hydroxyl tyrosol from 10 breeds of olive leaves; extracting by using ultrasonic wave or microwave; and carrying out macroporous resin adsorption and medium pressure column chromatogram separation to prepare an extract containing more than 90% of hydroxyl tyrosol. Compared with the traditional purifying method, the method has the characteristics of simplicity, convenience, quickness, economy, high yield, and the like and is suitable for industrial production.

Description

technical field [0001] The invention relates to the technical field of plant extracts, in particular to a method for efficiently enriching high-quality hydroxytyrosol from olive leaves. Background technique [0002] Olive (Olea europaea L.), as a world-renowned woody oil tree species, is widely planted in the Mediterranean region. Now this region has become the main growing area of ​​olives, accounting for about 98% of the world's olive tree planting area. [0003] The antioxidant bioactive components of olive oil are mainly oleuropein, hydroxytyrosol, flavonoids, lignans, and caffeoyl phenylethanoid glycosides. Small molecule diphenolic compounds with the parent structure of hydroxytyrosol have the most antioxidant activity, among which hydroxytyrosol and oleuropein are the hotspots of research at home and abroad. [0004] Natural hydroxytyrosol mainly exists in olive fruit, leaves and olive oil processing wastewater, especially the content of oleuropein and hydroxytyrosol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C39/11C07C37/70
Inventor 王成章叶建中董艳鹤原姣姣
Owner INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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