Method for preparing antibacterial compound

A compound and seed technology, applied in the field of bioengineering, can solve problems such as no treatment methods, and achieve the effect of easy separation and purification, simple process flow and high potency

Inactive Publication Date: 2011-02-23
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, topical therapy has been able to cure most patients with superficial mycosis, but there are still few drugs that can treat deep mycosis, and some diseases still have no effective treatment

Method used

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  • Method for preparing antibacterial compound
  • Method for preparing antibacterial compound
  • Method for preparing antibacterial compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0033] culture medium

[0034] (1) LB slant medium (%): yeast powder 0.5, peptone 1.0, NaCl 0.5, agar 2.0, adjust pH to 7.4-7.6.

[0035] (2) Seed medium (%): glucose 1.5, yeast powder 1.0, K 2 HPO 4 0.1, MgSO 4 0.05, FeSO 4 0.001, add CaCO after adjusting pH7.0 3 0.04.

[0036] (3) Fermentation medium (%): soybean powder 0.6, soluble starch 3.0, yeast powder 1.0, K 2 HPO 4 0.1, NaCl 0.2, MgSO 4 0.005, FeSO 4 0.002, pH 6.5.

[0037] (4) Candida albicans medium (%): glucose 2.0, peptone 1.0, agar 2.0.

[0038] Strain isolation and purification

[0039]Soil samples were cross-sampled at five points in the area demarcated in the East China Sea of ​​Zhejiang Province. Take 10g of soil samples at each point and put them into conical flasks. bottle, placed on a shaker and vibrated for 30 minutes, so that the bacteria in the soil sample were fully dispersed in the water, that is, 10 -1 bacteria liquid. After the inoculation loop was flame sterilized and cooled, di...

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Abstract

The invention discloses a method for preparing an antibacterial compound, which comprises the following steps of: inoculating bacillus subtilis (Bacillus subtilis) GMCC No.4140 into a fermentation medium, culturing for 12 to 60 hours, separating and purifying. The antibacterial compound has strong effect of inhibiting Candida albicans and better antibacterial effect than the conventional nystatin. The antibacterial compound has strong effect of inhibiting the Candida albicans and other yeast fungi, also has good effect on Aspergillus niger and other filamentous fungi, and simultaneously has better effect of inhibiting Escherichia coli and other bacteria. The antibacterial compound produced by the method has high potency, the technical flow is simple, and antibacterial substances are easy to separate and purify.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a preparation method of an antibacterial compound. Background technique [0002] In recent years, with the improvement of modern medical treatment, the survival rate of patients with cancer, AIDS, organ and bone marrow transplantation has increased greatly. Chemotherapy, venous catheterization, hemodialysis and other interventional treatments lead to decreased immunity, and are easily invaded by conditionally pathogenic fungi that are not originally pathogenic, causing deep mycoses (Systemic fungal infection). In recent years, this serious life-threatening fungal infection has shown a rapid growth trend, and the number of patients has continued to increase. [0003] Clinically, with the increase of fungal infection rate, the utilization rate of antifungal drugs has increased, and the drug-resistant strains have also increased, which brings certain difficulties to clinical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/08C12N1/20C12R1/125
Inventor 吴绵斌张书衍贺娟陈正杰
Owner ZHEJIANG UNIV
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