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Method for storing pear pollen germplasm for long term

A pollen and pollen collection technology, applied in the field of plant developmental biology, can solve the problems of reduced pollen vigor, failure to meet the needs of production and scientific research, and achieve the effects of simple operation, extensive practical value, and overcoming seasonal limitations

Inactive Publication Date: 2012-05-23
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the vigor of pear pollen decreases rapidly during conventional storage, so that it cannot meet the needs of production and scientific research

Method used

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  • Method for storing pear pollen germplasm for long term
  • Method for storing pear pollen germplasm for long term
  • Method for storing pear pollen germplasm for long term

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Embodiment Construction

[0018] (1) Experimental method

[0019] 1, pollen collection: this patent takes 'Fengshui' pear as the research test material, collects the big bud stage flower bud that is in bud, collects the anther, drys and pops powder at room temperature, wraps with paraffin paper after the pollen is scattered, according to different method to preserve pollen and conduct regular testing;

[0020] 2. Pollen preservation method:

[0021] (1) Gradient treatment of pollen storage temperature: Divide the same bag of pollen into five parts, each about 5cm×5cm×0.5cm in size, embedded with discoloration silica gel (a granular desiccant, blue, turns white after absorbing water) ), stored at room temperature, 4°C, -20°C, -73°C, and -196°C respectively. The pollen germination rate and pollen tube length were measured once every 5 days at room temperature according to the above-mentioned pollen culture method. Measured every 10 days at 4°C, and pollen stored at -20°C, -73°C, and -196°C will be mea...

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Abstract

The invention provides a method for reliably storing pear pollen germplasm for long term, belonging to the field of plant developmental biology. The method is characterized by putting the pear pollen into an allochroic silicagel box after drying the pear pollen, packing and sealing the box with tinfoil, freezing the pollen in liquid nitrogen and storing the pollen at ultralow temperature, placingthe stored pollen for 24 hours at minus 73 DEG C, minus 20 DEG C and 4 DEG C respectively in sequence to gradually revive the pollen when the pollen needs to be used in the coming year and finally restoring the pollen to room temperature in dark environment for later use. The method is secure and reliable, can effectively maintain the vigor of the pear pollen, is simple and easy to operate, saves space, can be used for reference to preserve other germplasm resources and has wide application value.

Description

1. Technical field [0001] The invention relates to a method for long-term storage of pear pollen germplasm, belonging to the field of plant development biology. 2. Background technology [0002] Pear (Pyrus) belongs to the gametophytic self-incompatibility fruit trees, and most of the varieties are self-pollinated and do not bear fruit. In production, pollination trees must be arranged, or artificial pollination can be performed to obtain the expected yield and quality. However, in production, it is often necessary to collect and transfer pollen from other places, or even to use pollen stored in the previous year, due to unforeseen flowering periods or weather reasons; Use pear germplasm pollen with special or excellent traits to enrich genetic diversity; in addition, because pear is a typical gametophytic self-incompatibility fruit tree, pear pollen often plays an important role in the study of self-incompatibility mechanism. However, pear pollen can only be harvested once...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01F25/00A01D91/04A01H1/02
Inventor 张绍铃陶书田吴俊齐永杰齐开杰吴华清
Owner NANJING AGRICULTURAL UNIVERSITY
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