Spruce somatic embryogenesis and plant regeneration method
A technology of embryogenesis and somatic cells, applied in plant regeneration, botany equipment and methods, horticultural methods, etc., can solve problems such as difficulty in cultivating organs, low plant regeneration rate, difficulty in meeting the needs of production and application, and achieve high reproduction coefficient, The effect of alleviating urgent needs and low cost
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Embodiment 1
[0038] (1) Preparation of Chinese fir immature seed embryos
[0039] The test materials were immature embryos of the hybrid combination of Chinese fir excellent trees, collected from the seed orchards of Chinese fir clones in Shunchang and Weimin, Fujian, respectively, and repeated sampling for 2 years. The cones are collected every 7 days from the end of June to the end of August every year. The seed development stages corresponding to each seed collection time point were the developmental stages from immature embryo to mature embryo. After the cones are collected, put them in a pre-frozen ice box on site and wrap them with moist gauze to prevent water loss; then quickly put them in a 4°C refrigerator for storage for later use. Store in refrigerator at 4°C for at least one week, which is conducive to the efficient induction of ESM. Before inoculation, take the spare cones out of the refrigerator, use a scalpel and tweezers to remove the well-developed immature seeds with se...
Embodiment 2
[0063] In step (3), the 2 in the maintenance solid medium formula, 4-D is 2mg / L, 6-BA is 0.5mg / L, and the 2 in the liquid proliferation medium formula, 4-D is 0.5mg / L; In step (5), the ABA in the maturation medium formula is 8 mg / L, GA is 1 mg / L, and PEG 8000 is 200 g / L. Other reaction steps and conditions are the same as those in Example 1, and the test results show that the improved seedlings can be efficiently regenerated.
Embodiment 3
[0065] In step (3), the 2 in the maintenance solid medium formula, 4-D is 0.5mg / L, 6-BA is 0.2mg / L, and the 2 in the liquid proliferation medium formula, 4-D is 2mg / L; In step (5), the ABA in the maturation medium formula is 3mg / L, GA is 5mg / L, and PEG 8000 is 100g / L. Other reaction steps and conditions are the same as in Example 1, and the test results show that the seedlings of good species can be efficiently regenerated.
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