Rapid bulb multiplication method by using fritillaria cirrhosa leaves as explant

A kind of fritillary fritillary and explant technology, applied in the direction of plant cells, etc., can solve the problems such as the shortage of bulb resources cannot be quickly alleviated, the consumption of bulbs, the increase of bulb culture costs, etc. strong effect

Active Publication Date: 2011-04-06
薛永新
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing bulb tissue culture method all is to use bulb as explant to cultivate, because bulb itself is used as medicine part, and resource is limited, so use bulb as explant to cultivate bulb,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Induction of callus: choose healthy young and tender fritillary fritillaria plant leaves as explants, wash it with washing powder liquid and rinse it under running water for 30 minutes, then rinse it with 2.0% sodium hypochlorite solution for 15 minutes, use After washing and drying with clean water, put them into 0.1% mercuric chloride solution for disinfection for 5 minutes, then rinse with sterile water for 3 times, and dry the surface moisture with sterile filter paper, and then cut the treated plant leaves on the ultra-clean workbench. into a size of about 2cm, and insert MS+6-BA2.0mg·L into the wound -1 +IAA0.1mg·L -1 + sucrose 30g·L-1+ agar 6.0~7.0g·L -1 In the culture medium, culture is carried out under the conditions of 20-21°C, light intensity of 1000-1800 lx, and 12 hours of light per day, and a large number of callus can be induced by culturing for 35-45 days;

[0018] (2) callus proliferation: select the yellow-green callus with fast growth rate in (...

Embodiment 2

[0022] (1) Induction of callus: select the young leaves of Fritillaria sichuanensis tissue culture seedlings growing healthy as explants, cut them into a size of about 2 cm on the ultra-clean workbench, and insert MS+6- BA2.0mg·L -1 +IAA0.5mg·L -1 + sucrose 30g·L-1+ agar 6.0-7.0g·L-1 medium, cultured at 20-21°C, light intensity 1000-1800lx, 12 hours of light per day, cultured for 35-45 days Induce a large amount of callus;

[0023] (2) callus proliferation: select the yellow-green callus with fast growth rate in (1) step, cut it into about 2g weight on the ultra-clean workbench and insert it in the 35g medium, the callus Proliferation medium is MS+6-BA2.0mg·L -1 +NAA0.5mg·L -1 + sucrose 30g·L -1 +Agar 6.5g·L -1 , cultured under the conditions of 20°C, light intensity of 1200lx, and 12 hours of light per day, after 28 days of culture, the callus can increase by 3.2 times;

[0024] (3) callus differentiation bulb: select the light yellow and yellow callus that grows tight...

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PUM

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Abstract

The invention discloses a rapid bulb multiplication method by using fritillaria cirrhosa leaves as an explant, comprising the steps of: inducing the callus; multiplying the callus; differentiating a small bulb; and germinating the small bulb. The adopted medium for inducing the callus comprises 1.0-2.0mg.L-1 of MS+6-BA, 0.1-0.5mg.L-1 of IAA, 30-50g.L-1 of sucrose, and 6.0-7.0g.L-1 of agar; the medium for multiplying the callus comprises 2.0mg.L-1 of MS+6-BA, 0.5-1mg.L-1 of NAA, 30-50g.L-1 0f sucrose, and 6.0-7.0g.L-1 of agar; the medium for differentiating the bulb comprises 2.0mg.L-1 of MS+6-BA, 0.1-0.4mg.L-1 of NAA, 30-50g.L-1 0f sucrose, and 6.0-7.0g.L-1 of agar; and the medium for germinating the small bulb comprises 2.0mg.L-1 of MS+6-BA, 0.5mg.L-1 of NAA, 30-50g.L-1 of sucrose, and 6.0-7.0g.L-1 of agar. Through the rapid bulb multiplication method by using fritillaria cirrhosa leaves as the explant, the fritillaria cirrhosa bulb can be multiplied abundantly and rapidly with low cultivation cost and high multiplication rate.

Description

technical field [0001] The invention relates to a method for multiplying bulbs, in particular to a method for rapidly multiplying bulbs by using fritillary fritillaria plant leaves as explants. Background technique [0002] The rare and endangered medicinal material Fritillaria cirrhosa D.Don is a plant of the Liliaceae family. It grows at an altitude of 3500m-4500m and is mainly produced in Sichuan, Tibet, Yunnan and other provinces. Chuan Fritillaria likes cold and cool climate conditions, and has the characteristics of cold resistance, humidity, high temperature, and shade. When the temperature reaches 30°C or the ground temperature exceeds 25°C, the plants will wither and cannot survive in areas with low altitude and high temperature. Chuan Fritillaria has the effect of clearing away heat and nourishing the lungs, reducing phlegm and relieving cough. It is used for symptoms such as dry cough due to lung heat, dry cough with little phlegm, overworked cough due to yin def...

Claims

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Application Information

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IPC IPC(8): C12N5/04
Inventor 薛永新代勇王跃华闫胜杰徐世军孙雁霞王晓蓉何宗晟
Owner 薛永新
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