Algal culture production, harvesting, and processing

A culture and nutrient technology, applied in seaweed cultivation, botanical equipment and methods, biochemical equipment and methods, etc., can solve problems such as multiplication

Inactive Publication Date: 2011-04-27
AQUATIC ENERGY LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are several obstacles to the production of these products from algae, including selection of suitable algae, development of suitable growth conditions for optimal lipid production, and prevention of contamination by undesired algal species and other organisms
Weather and pollution are constant threats and these hurdles are multiplied when pursuing large-scale cultivation of algae in outdoor locations

Method used

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Examples

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Effect test

Embodiment 1

[0048] This example demonstrates the cultivation of Chlorella cultures according to the present invention while maintaining culture selectivity. Scenedesmus oblique cultures (University of Texas) were used. To increase volume, slant cultures (20 mL, 500,000 cells / mL) were subcultured into 6 tubes (50 mL cultures until a concentration of 1 million cells / mL was reached) using UTEX nutrient medium, although other suitable medium. UTEX nutrient medium is a peptone medium of Bristol medium containing 1 g / L peptone. Bristol medium is 2.94mM NaNO 3 , 0.17mM CaCl 2 2H 2 O, 0.3mM MgSO 4 ·7H 2 O, 0.43mM K 2 HPO 4 , 1.29mM KH 2 PO 4 , and 0.43mM NaCl. Once growth was established, the cultures were transferred to 250ml Erlenmeyer flasks and nutrient concentrations were initiated from that point on, and these concentrations are described below. When the cell density increases (to a concentration of 1 million cells / ml in a 200 ml culture), transfer the culture to a 1.5 L bubble ...

Embodiment 2

[0057] This example demonstrates the cultivation of target algal cultures for the production of β-carotene according to the present invention. β-Carotene is a lipid- and oil-soluble product that has antioxidant, free radical-scavenging properties and cancer-preventing activity. Different algal species can be cultured to obtain beta-carotene globules. For example, seawater, sometimes freshwater, algae of the genus Dunaliella, such as Dunaliella salina, D. parva, D. viridis, and any combination thereof, may be employed in the basal medium. Dunaliella is a unicellular, diflagellated Euglena. Dunaliella parva (D.parva) and Dunaliella salina can accumulate large amounts of β-carotene. These algae can grow in the range of 20 to 40°C, but can tolerate much lower temperatures as well.

[0058] The following materials can be used to prepare media for algal β-carotene production: 2.14M NaCl, 4.81 μM FeCl 3 , 1.82 μM MnCl 2 , 0.13mM NaH 2 PO 4 , and 1.18mM NaNO 3 , seawater and o...

Embodiment 3

[0060] This example demonstrates the cultivation of diatom or green algae cultures for aquaculture feed according to the invention. Diatoms, Skeletons costas, Chaetoceros calcareous, Prymnesiophycean Isochrysis galpana and Prasinophycean Tetraselmis suecica can be grown in outdoor ponds for the production of aquaculture feed . Storage cultures were maintained under a constant light of 2000 lux, at a temperature of 22-24°C. containing NaNo 3 , NaH 2 PO 4 、Na 2 SIOs 3 , FeCl 3 and Na 2 Diatoms were grown in EDTA seawater medium. For green algae, omit the silicate solution. Stock cultures were maintained in the laboratory and cultures were inoculated into outdoor ponds. The optimum temperature is 20 to 33°C. The algae are harvested using a 20 micron filter and the biomass is air-dried to feed shrimp, mussels and other fish larvae. Products include not only aquaculture feed, but often protein and fibre.

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Abstract

Materials and methods are provided for growing algae while maintaining culture selectivity. Algae that can be grown include, for example, green algae such as those of the genus Scenedesmus. Lipid obtained from the algae can be used to produce biofuels such as biodiesel or polyunsaturated fatty acids such as omega-3 fatty acids. Feedstocks such as animal feed and aquaculture feed can also be produced as can phytonutrients such as asataxanthin and beta-carotene.

Description

[0001] This application claims priority to US Provisional Patent Application 61 / 023,572, filed January 25, 2008, which is hereby incorporated in its entirety. Background technique [0002] Growing global demands and environmental concerns have led to the search for alternative and greener sources of fuels, animal feed, pharmaceuticals, nutraceuticals, polyunsaturated fatty acids, plant nutrients, minerals, vitamins and other products. One environmental source of these products is algae. Algae are a particularly attractive source because algae can use land not normally available for food production or other purposes to grow. However, there are several obstacles to the production of these products from algae, including selection of suitable algae, development of suitable growth conditions for optimal lipid production, and prevention of contamination by undesired algal species and other organisms. These hurdles are multiplied when pursuing large-scale cultivation of algae in out...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K63/06C12M1/00C12N1/12A23L35/00
CPCC12P7/6463A01G33/00C12P23/00Y02E50/13C12P7/6427C12N1/12C12P7/649C12P7/6472Y02A40/80Y02E50/10C12P7/6458C12P7/6434C12P7/6432
Inventor 普里西利亚·拜耳·德马里斯拉曼努金·拉维库马尔菲利普·旺德维尔
Owner AQUATIC ENERGY LLC
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