LAMP (loop-mediated isothermal amplification) primer group for detecting transgenic corn strain MIR604 at normal temperature

Abstract

The invention relates to an LAMP (loop-mediated isothermal amplification) nucleic acid primer for detecting a transgenic corn strain MIR604 and carrying out normal temperature amplification. The LAMP nucleic acid primer for amplification comprises an FIP (forward inner primer), an F3 primer, a BIP (backward inner primer), a B3 primer, an LF primer and an LB primer. An LAMP detection system has high specificity and sensitivity, can rapidly, conveniently and efficiently detect the transgenic corn strain MIR604 and meets the requirements of a port and a base lab on the detection of the transgenic corn strain MIR604.

Description

technical field [0001] The invention relates to the field of detection of transgenic corn strains, provides the detection of transgenic corn strain MIR604 by using normal temperature amplification technology, and is suitable for application in port inspection and quarantine institutions. Background technique [0002] With the rapid development of genetically modified technology, the industrialization speed of global agricultural biotechnology has also been greatly accelerated, and the promotion and planting area of ​​genetically modified crops has been increasing year by year. A cautious attitude is generally adopted in this regard, and a series of regulations have been introduced to restrict the trade of genetically modified products, requiring the labeling of genetically modified crops and their processed products. Therefore, it is particularly important to establish a sound genetically modified agricultural product and food testing system. [0003] There are many kinds o...

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Problems solved by technology

[0004] Because ordinary PCR method and real-time fluorescent PCR detection method require temperature-changing process and corresponding rapid heating and cooling and temperature control, they also need expensive special-purpose PCR equipment, expensive instruments and equipment, and serious pollution problems (high false positive rate), Limit the promotion of PCR detection method

On-site testing requires less dependence on instruments. The isothermal amplification method can solve the defects and deficiencies of the current high degree of dependence on instruments for the safety testing of genetically modified organisms and meet the needs of on-site testing.

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