Research and application of electrochemiluminescent immunosensor for detecting tumor markers

A tumor marker and electrochemistry technology, applied in the field of diagnosing tumor markers, can solve the problem of low sensitivity

Inactive Publication Date: 2011-05-25
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 2. The electrochemical immunoassay method is convenient and fast, and because the potential is controllable, the selectivity i

Method used

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  • Research and application of electrochemiluminescent immunosensor for detecting tumor markers
  • Research and application of electrochemiluminescent immunosensor for detecting tumor markers
  • Research and application of electrochemiluminescent immunosensor for detecting tumor markers

Examples

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preparation example Construction

[0031] A preparation method of an electrochemiluminescence immunosensor for detecting tumor markers, which is characterized by comprising the following steps:

[0032] (1) Select tumor markers with a higher clinical incidence for determination;

[0033] (2) Prepare a quantum dot nanomaterial solution;

[0034] (3) Using assembly surface modification technology, etc., the antibody is modified on the electrode surface to make an electrochemiluminescence immunosensor.

[0035] The modification of the antibody of the present invention to the surface of the immunosensor and the determination of tumor markers includes the following steps:

[0036] (1) Clean the electrodes with deionized water, acetone and ethanol ultrasonically, and then use high-purity N 2 Blow dry

[0037] (2) Immerse the electrode obtained in step (1) in a 4% NaOH solution for 10-30 minutes, then rinse with deionized water and dry at 120°C for 1-2 hours;

[0038] (3) Adsorb the specific antibody (Ab) to the surface of the tr...

Embodiment 1

[0046] Example 1 (Embryonic antigens, such as alpha-fetoprotein)

[0047] A preparation method of an electrochemiluminescence immunosensor for detecting alpha-fetoprotein, including the following steps:

[0048] (1) Select alpha-fetoprotein with higher clinical incidence for determination;

[0049] (2) Using assembly technology, modify the alpha-fetoprotein antibody to the electrode surface to make an electrochemiluminescence immunosensor;

[0050] (3) ITO electrode is used as the working electrode, and the electrode is cleaned with deionized water, acetone and ethanol ultrasonically, and then with high-purity N 2 Blow dry

[0051] (4) Immerse the processed electrode in 4% NaOH solution for 10 minutes, then rinse with deionized water, and dry at 120°C for 2 hours;

[0052] (5) Preparation of CdTe quantum dot solution: in N 2 Under the protection, the prepared NaHTe is the Te precursor, and CdCl 2 In the reaction, with thioglycolic acid as a stabilizer, a water-soluble CdTe quantum dot so...

Embodiment 2

[0061] Example 2 (carbohydrate markers, such as CA19-9)

[0062] A preparation method of an electrochemiluminescence immunosensor for detecting CA 19-9, including the following steps:

[0063] (1) Select CA19-9 with higher clinical incidence for determination;

[0064] (2) Using assembly technology, modify the CA19-9 antibody to the electrode surface to make an electrochemiluminescence immunosensor;

[0065] (3) ITO electrode is used as the working electrode, and the electrode is cleaned with deionized water, acetone and ethanol ultrasonically, and then with high-purity N 2 Blow dry

[0066] (4) Immerse the processed electrode in 4% NaOH solution for 10 minutes, then rinse with deionized water, and dry at 120C for 1 hour;

[0067] (5) Preparation of CdTe quantum dot solution: in N 2 Under the protection, the prepared NaHTe is the Te precursor, and CdCl 2 In the reaction, with thioglycolic acid as a stabilizer, a water-soluble CdTe quantum dot solution is prepared;

[0068] (6) Take 20 μl ...

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Abstract

The invention discloses a method for researching and applying an electrochemiluminescent immunosensor for detecting tumor markers. An electrode preparation method (shown in attached drawings as schematic diagrams) comprises the following steps of: selecting a tumor marker with higher clinical morbidity for detection; preparing a quantum dot nano-material solution; and preparing the electrochemiluminescent immunosensor by modifying an antibody onto an electrode surface by using an electrode surface modification technology. A method for detecting the tumor markers comprises the following step of connecting a modified electrode to an electrochemiluminescent instrument so as to detect a tumor marker in a sample. The electrode provided by the invention has strong specificity and high sensitivity and can reach ng level; and moreover, the time required for completing a basic detection process is short, and the cost is low. The method for detecting the tumor markers through the electrode has the advantages that: the operation is fast and simple, and both reactions and results are automatically completed and recorded by the instrument.

Description

Technical field [0001] The invention relates to the technical field of tumor marker detection, and more specifically to the preparation of an electrochemiluminescence immunosensor for detecting tumor markers. The invention also relates to the use of the electrochemiluminescence immunosensor to diagnose tumor markers. Methods. Background technique [0002] Tumors are the killer of human health. Malignant tumors, also called cancers, are a type of disease that seriously threatens human health and life. It is caused by the body's various internal or external carcinogenic factors, and cells in local tissues lose their normal growth at the genetic level. Regulation, resulting in the formation of new organisms from abnormal growth. The occurrence of tumor is a long-term, multi-stage, multi-gene change accumulation process, with the complexity of multi-factor regulation and multi-gene control. The rising trend of tumor morbidity and mortality directly threatens human health, and its ...

Claims

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Application Information

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IPC IPC(8): G01N33/532G01N21/66
Inventor 于京华张萌万夫伟颜梅葛慎光藏德进戴平高伟强
Owner UNIV OF JINAN
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