Petroleum ether extract of traditional Chinese medicine for preventing and treating glucose and lipid metabolic disturbance and preparation method thereof

A technology of petroleum ether extraction and glucose and lipid metabolism, which is applied in food preparation, metabolic diseases, drug combination, etc., can solve the problems that product quality is difficult to obtain effective control, affects the stability of clinical efficacy of products, and the preparation of pharmaceutical preparations cannot be diversified. To achieve the effect of safety, no obvious toxic and side effects, controllable product quality, and widen application

Active Publication Date: 2013-08-21
QINGDAO BAILI CAIXIN MEDICAL TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although Fufang Zhenzhu Tiaozhi Decoction has good blood lipid regulation and anti-arteriosclerosis functions and clinical curative effects, but because it is a compound preparation of traditional Chinese medicine, there are still many shortcomings: (1) Its ingredients are complicated, and its active ingredients are not clear enough, It is not easy to quantify, and the quality of its products is not easy to be effectively controlled in practical applications, which will eventually affect the stability of the clinical efficacy of the product; (2) The preparation process is relatively simple, the active ingredients in the prescription cannot be purified as they should be, and there are many impurities. Large (nearly more than 5 grams per day), the patient's compliance is not ideal; (3) due to the large amount of prescription extracts, it is inconvenient to make various controlled-release dosage forms, the preparation of pharmaceutical preparations cannot be diversified, and the scope of application is subject to various restrictions

Method used

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  • Petroleum ether extract of traditional Chinese medicine for preventing and treating glucose and lipid metabolic disturbance and preparation method thereof
  • Petroleum ether extract of traditional Chinese medicine for preventing and treating glucose and lipid metabolic disturbance and preparation method thereof
  • Petroleum ether extract of traditional Chinese medicine for preventing and treating glucose and lipid metabolic disturbance and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation of embodiment 1 Chinese medicine compound total extract

[0028] The two medicines of Ligustrum lucidum, Atractylodes macrocephala, Eucommia ulmoides, Panax notoginseng, Daji, Salvia miltiorrhiza, Coptis chinensis, and Bergamot (weight ratio 2:1:1:1:1:2:1:1) Crushed into coarse powder, reflux extraction with 60% ethanol of 10 times, 8 times and 6 times the amount of the medicinal material respectively, 2 h each time, combine the extracts, recover the ethanol and make the concentration of the alcohol extraction part equal to 1 ml 1 g crude drug; the rest of the medicinal materials in the prescription were decocted and extracted 3 times with 12 times, 10 times and 8 times the amount of water respectively, each time for 2 hours, combined 3 times of water extracts, and concentrated under vacuum to 1 g medicinal materials / ml extract solution, the water body part and the previous alcohol extraction part were combined to obtain the total extract of the tradi...

Embodiment 2

[0029] Example 2 Preparation of Petroleum Ether Extraction Parts of Compound Total Extract

[0030] Extract once with petroleum ether of 10 times the volume of the total compound extract, and separate the petroleum ether layer; then extract once each with 8 and 6 times the volume of petroleum ether, separate and obtain the petroleum ether layer, combine the petroleum ether extracts for 3 times, and recover Petroleum ether, then vacuum-dried at 0.08MPa and 65°C to obtain petroleum ether extract (FTZEE);

[0031] The yield of the extract was calculated according to the total dosage of the compound Chinese medicine and the amount of the extracted part. The yield of petroleum ether extract is 0.3%-0.5% (3.0-5.0g of petroleum ether extract is obtained from 1kg crude drug).

Embodiment 3

[0032] Example 3 Chemical Composition Analysis of the Petroleum Ether Extraction Part of the Compound Total Extract

[0033] Embodiment 2 compound petroleum ether extract (FTZEE), through HPLC analysis, obtains figure 1 sample fingerprint.

[0034] UPLC chromatographic conditions for FTZ petroleum ether fraction: Agilent HC-C 18 Chromatographic column (4.6mm×250mm, 1μm), guard column phenomenonex KJO-4282-C 18 Column; mobile phase using acetonitrile (A) - water (containing 0.25% glacial acetic acid + 0.13% triethylamine) (B) binary gradient system. Gradient elution: 2~5% A (0~5 min), 5%~20% A (5~35 min), 20% A (35~45 min), 20%~35% A (45~70 min ), 35%~80%A (70~85 min), 80%~100%A (85~87 min), 100%A (87~89 min). The detection wavelengths were 254 nm, 300 nm and 330 nm, the column temperature was 30 °C, the flow rate was 0.2 mL / min, and the injection volume was 10 μL.

[0035] Qualitative analysis results: FTZ petroleum ether mainly contains waxy alcohol, β-sitosterol, n-he...

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Abstract

The petroleum ether extract of traditional Chinese medicine for the prevention and treatment of sugar and lipid metabolism disorders, wherein the active ingredients of the extract are composed of 1-cerotol, ß-sitosterol, n-hexacosanoic acid, atractylenolide III, oleanolic acid, berberine, jateorrhizine, salvianolic acid B, cyclotetracosane, 9,12-octadecadienoic acid, 5,7-dimethoxycoumarin, ginsenoside Rb1. The preparative method comprises extracting crude drug Radix Salviae Miltiorrhizae, Fructus Ligustri Lucidi, Rhizoma Coptidis, Herba Cirsii Japonici, Cortex Eucommiae, Rhizoma Atractylodis Macrocephalae, Radix Notoginseng and Fructus Citri Sarcodactylis with C1-3 alcohol and / or water, combining total extracts, extracting the total extracts with petroleum ether to obtain the petroleum ether extract.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicines, in particular to a traditional Chinese medicine petroleum ether extract for preventing and treating diseases related to glucose and lipid metabolism disorders (hyperlipidemia, diabetes, metabolic syndrome, animal atherosclerosis and other related diseases) and a preparation method thereof. Background technique [0002] Chinese patent 200410051250.4 discloses a drug for treating hyperlipidemia, named "Fufang Zhenzhu Tiaozhi Fang" (FTZ), which contains Ligustrum lucidum, Daji, Atractylodes macrocephala, Salvia miltiorrhiza, Eucommia, bergamot, and notoginseng and coptis. The preferred weight percentage of each component is Ligustrum lucidum 20-25%, Daji 12-15%, Atractylodes macrocephala 10-15%, Salvia miltiorrhiza 10-15%, Eucommia 10-15%, Bergamot 10-12%, Panax notoginseng 10% ~ 12%, coptis 6 ~ 10%. This prescription is used to treat hyperlipidemia and has achieved ideal curative effe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/704A61K31/575A61K31/56A61K31/4375A61K31/365A61K31/352A61K31/343A61K31/201A61K31/20A61K31/045A61K31/015A61P3/06A61P3/10A61P3/00A61P9/10A23L1/30
CPCA61K36/537A61K36/638A61K36/46A61K36/718A61K36/28A61K36/284A61K36/752A61K36/258A61K31/44A61K45/06A61P3/00A61P3/06A61P3/10A61P9/10A61K2300/00
Inventor 郭姣贝伟剑
Owner QINGDAO BAILI CAIXIN MEDICAL TECH CO LTD
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