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Polarization imaging nondestructive detection device

A non-destructive detection and polarization imaging technology, applied in measurement devices, material analysis by optical means, instruments, etc., can solve the problem of inability to obtain the collagen type ratio of epithelial tissue and the directional information of collagen, etc., and achieve high contrast and high resolution. rate effect

Inactive Publication Date: 2011-06-15
FUJIAN NORMAL UNIV
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Problems solved by technology

However, it cannot obtain information on the proportion of collagen types in epithelial tissue and the orientation of collagen

Method used

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  • Polarization imaging nondestructive detection device
  • Polarization imaging nondestructive detection device
  • Polarization imaging nondestructive detection device

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Embodiment Construction

[0016] In order to have a further understanding of the present invention, the following in conjunction with the attached figure 2 Embodiments of the present invention are described in detail.

[0017] figure 2 Middle, 1. laser; 2. polarizer; 3. X-Y scanning system; 4. convex lens A; 5. convex lens B; 6. rotating stage; 7. objective lens; 8. dichroic mirror; 9. wave chip, Analyzer and related electromagnetic control unit; 10. Condenser B; 11. Filter; 12. Condenser A; 13. Photomultiplier tube A; 14. Computer; 15. Photomultiplier tube B.

[0018] The light beam emitted by the tunable wavelength laser 1 passes through a polarizer 2 to obtain oriented linearly polarized light. Then it is transmitted to the X-Y scanning system 3 and the convex lens A4 and convex lens B5 in the imaging magnification system, and then the light passing through a dichroic mirror 8 is focused by the objective lens 7 onto the sample on the rotating stage 6 . The second harmonic signal generated by th...

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Abstract

The invention relates to a polarization imaging nondestructive detection semi-automatic device. The device is characterized by comprising a laser, a polarization detection system, a light path transmission system, an object stage, a detection system and a calculation processing system; the laser beam emitted by the laser sequentially passes through the polarization detection system, the light path transmission system and the object stage; the laser beam can generate secondary harmonic signal light and fluorescent signals when irradiating the sample on the object stage, then returns to the light path transmission system and the polarization detection system, and arrives at the detection system; the detection system and the light path transmission system are controlled by a computer; and the polarization detection system and the object stage are controlled manually. The device can rapidly and nondestructively detect materials with birefringence effect, such as wafer, biological tissues and plants in real time, and can obtain high-resolution and high-contrast images and the intensity and direction of the images.

Description

technical field [0001] The invention relates to a non-destructive detection device for polarization imaging, which can be used to distinguish the fine structure of birefringent biological tissue and other birefringent substances. fields such as computer technology and medicine. Background technique [0002] For the methods of ultrafine structure of biological tissue, there are mainly methods such as electron microscopy, X-ray, biochemical analysis, histopathological analysis, and multiphoton fluorescence technology. Among them, electron microscopy, X-ray, biochemical analysis, histopathological analysis and other methods have the biggest disadvantage of serious damage and cannot be detected in real time and in vivo. Although the multiphoton fluorescence technology combined with multiphoton microscopy can obtain high-resolution, high-contrast microstructures in real time without loss, it has very high peak power and low average power, reducing or eliminating photobleaching a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/23G01N21/64
Inventor 吴淑莲李晖章小曼李志芳
Owner FUJIAN NORMAL UNIV
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