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Compositions and methods for improving plants

A plant and biomass technology, applied in botany equipment and methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of identification and cloning fragmentation, incompleteness, lack of evidence, etc.

Inactive Publication Date: 2011-06-15
VIALACTIA BIOSCI NZ
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Although some potentially useful genes have been identified, the identification and cloning of plant genes conferring stress tolerance remains fragmented and incomplete
Although stress-induced proteins are postulated to have a role in stress tolerance, evidence is lacking and the functions of many of these stress-responsive genes are unknown

Method used

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  • Compositions and methods for improving plants
  • Compositions and methods for improving plants
  • Compositions and methods for improving plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0414] Example 1: Identification of polynucleotides that modulate biomass production and tolerance to environmental stress

[0415] introduce:

[0416] Perennial ryegrass (Lolium perenne L.) is a cool-temperate forage grass from the families Poaceae and Festucaceae. To generate a relative gene expression-like profile in ryegrass, pre-grazed and post-grazed plants obtained from ambient temperature-grown, cold-grown, hydrated, dehydrated, and rehydrated or dehydrated in autumn, summer, spring, and winter RNA was extracted from the samples obtained in and used to construct a SAGE (Serial Analysis of Gene Expression) (Velculescu et al. 1995, Science 270:484-487) library.

[0417] Materials and methods

[0418] The cultivated variety (cv.) Bronsyn of perennial ryegrass (Lolium perenne L.) was used throughout this study. Field grown samples were collected from active paddocks at Dexcel in Hamilton, New Zealand during the peak of each season. Grass samples were collected from pre...

Embodiment 2

[0445] Example 2: Identification of ORF136 variants

[0446] Using the ORF136 polynucleotide sequence as the seed sequence pair i) GenBank Nucleotide Acquisition NR / NT database (v2.2.17 release date August 26, 2007) and ii) Patent Sequence Database (v2.2.17 release date August 2007 26) Perform a discontinuous large comparison (megablast) BLASTN search.

[0447] The polypeptide sequence encoded with ORF136 was used as the seed sequence for position-specific repeated BLASTP searches against the GenBank NR database (v2.2.17 release date 26 August 2007). A USPTO search was also performed on the UniRef100 protein database at EBI (v2.2.15 release date October 15, 2006). This gene appears to encode a protein similar to rice stress-related protein genes with low homology as described (Mukhopadhyay et al., 2004, PNAS (USA) 101(16):6309-6314).

[0448] Variant sequences were aligned using the EMBOSS tool EMMA (Thompson, J.D., Higgins, D.G. and Gibson, T.J. 1994, CABIOS, 10, 19-29.), a...

Embodiment 3

[0454] Example 3: Preparation of vectors containing polynucleotides of the present invention for plant transformation and transformed plants

[0455] Vectors containing ORF136 (CORF136 and DORF136)

[0456] Vectors for overexpression of ORF136 were prepared by standard molecular biology techniques. The vector in which ORF136 was driven by a double 35S promoter was designated as CORF136. The vector in which the ryegrass dehydrin-like promoter drives ORF136 was designated as DORF136.

[0457] (CORF136) plots are shown in figure 1 middle. The sequence of CORF136 is shown in image 3 Neutralizes SEQ ID NO:20.

[0458] The plot of DORF136 is shown in figure 2 middle. The sequence of DORF136 is shown in Figure 4 and SEQ ID NO:21.

[0459] Transformation of plants with polynucleotides of the invention

[0460]Perennial ryegrass (Lolium perenne L. cv. Impact) was transformed essentially as described by Bajaj et al. (Plant Cell Reports, 2006, 25:651-659). Embryogenic calli...

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Abstract

The invention provides an isolated polynucleotide encoding a polypeptide with the sequence of SEQ ID NO: 1 or a variant thereof, wherein the variant is a polypeptide capable of modulating in a plant at least one of: i) biomass, ii) seed yield, and iii) tolerance to at least one environmental stress selected from dought, cold, freezing, heat and salinity. The invention also provides construct, vectors, host cells, plant cells and plants genetically modified to comprise the polynucleotide. The invention also provides methods for producing and selecting plants that are altered for at least one of: i) biomass, ii) seed yield, and iii) tolerance to at least one environmental stress selected from dought, cold, freezing, heat and salinity, making use of the polynucleotides of the invention.

Description

technical field [0001] The present invention relates to compositions and methods for preparing plants with improved biomass and / or seed yield and / or stress tolerance. Background technique [0002] As the world population grows, a major goal of agricultural research is to improve the biomass yield and seed yield of crop and forage plant varieties. [0003] Until now such improvements have relied on selectively breeding plants for desired traits. But for many plants, the hybrid genetic complements produced in the progeny do not produce the same desired traits as their parents, thus limiting the effectiveness of selective breeding methods. [0004] Advances in molecular biology now make it possible to genetically manipulate plant and animal germplasm. Genetic engineering of plants involves isolating and manipulating genetic material and subsequently introducing such material into plants. This technology has developed plants capable of expressing drugs and other chemicals, pl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29A01H5/10C07K14/415A01H5/00C07H21/04C12N15/82
CPCC07K14/415C12N15/8261Y02A40/146
Inventor S·普蒂加埃C·J·布赖恩特S·巴贾杰K·R·坦普尔顿
Owner VIALACTIA BIOSCI NZ