Inoculation method for tissue culture of Rieger Begonia

Abstract

The invention belongs to the field of plant tissue culture, particularly to the field of the tissue culture of the Rieger Begonia. The inoculation method for the tissue culture of the Rieger Begonia is characterized in that a flattened young leaf on a Rieger Begonia plant is selected, cleaned and disinfected; the edge of the leaf is cut off, and the leaf is cut to be 0.6 to 1.5 cm in size; the Rieger Begonia leaf is inoculated into an induction culture medium in a reverse manner (the upper surface of the leaf is clung to the culture medium); and the inoculated leaf is cultivated rotationally. The inoculation method for the tissue culture of the Rieger Begonia has the advantages that the explant of the Rieger Begonia leaf can absorb nutrient and water more easily, the browning rate is low, the enlargement ratio is high, the growth potential of the leaf is improved, and the growth condition of the leaf is stable.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, in particular to the field of Rieger crabapple tissue culture. Background technique [0002] Rieger begonia (Rieger begonia) is a perennial herbaceous flower of the genus Begonia in the Begoniaceae family (Regoniaceaceae). It is a hybrid strain of bulbous begonias and wild begonias. Rieger Begonia has rich flower shapes and colors, most of which are double petals, and the flowering period lasts for more than half a year, especially when the flowering period coincides with New Year's Day and Spring Festival. It has extremely high ornamental value and economic value. However, Rieger Begonia has the disadvantages of few seeds after flowering, no tubers, and low survival rate of stem cuttings, which is not conducive to mass reproduction. Although tissue culture can obtain a large number of seedlings in a short period of time, it reduces production costs and is commercialized. Production provides ...

AI Technical Summary

Problems solved by technology

However, Rieger Begonia has the disadvantages of few seeds after flowering, no tubers, and low survival rate of stem cuttings, which is not conducive to mass reproduction. Although tissue culture can obtain a large number of seedlings in a short period of time, it reduces production costs and is commercialized. Produce high-quality seedlings

However, the growth effect is uneven and the growth is uneven, which affects the value created by production

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