Method for comprehensively utilizing rice bran

A technology of rice bran and defatted rice bran, which is applied to the preparation method of peptide, chemical instrument and method, application and other directions, can solve the problems of affecting the physiological activity of the product, the difference in molecular weight distribution is large, and the salt content of the finished product is high.

Active Publication Date: 2011-07-06
武汉百信正源生物技术工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current process of preparing rice bran peptides by enzymatic hydrolysis, a large amount of lye or acid solution is added to adjust the pH of the solution during the enzymatic hydrolysis reaction, and there is no desalination pro...

Method used

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  • Method for comprehensively utilizing rice bran
  • Method for comprehensively utilizing rice bran

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method for comprehensively utilizing rice bran, the method comprising the steps of:

[0036] (1) Weigh 100g of rice bran, crush it through a 60-mesh sieve, use Soxhlet extraction method, use 95% n-hexane solvent to extract at 50°C for 2 hours, then filter the n-hexane solution, and dry the rice bran until the n-hexane is completely Volatility is defatted rice bran; the filtrate is completely evaporated with n-hexane with a rotary evaporator, and the rest is rice bran oil, weighing 11.3g;

[0037] (2) Pour the defatted rice bran in step (1) into 600mL water, heat to 45°C, adjust the pH to 9.0 with NaOH reagent, stir continuously for 15min, centrifuge at 3000r / min for 15min, and take the supernatant; Precipitated and dried, it was rice bran crude fiber, weighing 53.2g;

[0038] (3) Heat the supernatant of step (2) to 80°C, adjust the pH to 3.5 with citric acid reagent, stir continuously for 10 minutes, 3000r / min, 15 minutes, centrifuge to collect the precipitate, w...

Embodiment 2

[0048] A method for comprehensively utilizing rice bran, the method comprising the steps of:

[0049] (1) Weigh 500g of rice bran, crush it through a 60-mesh sieve, use Soxhlet extraction method, extract it with 95% n-hexane solvent at 50°C for 2 hours, then filter the n-hexane solution, and air-dry the rice bran until the n-hexane is completely Volatility is defatted rice bran; the filtrate is completely evaporated with n-hexane with a rotary evaporator, and the rest is rice bran oil, weighing 58.9g;

[0050] (2) Pour the defatted rice bran in step (1) into 2.5L of water, heat to 50°C, adjust the pH to 8.5 with NaOH reagent, stir continuously for 15min, centrifuge at 3000r / min for 15min, and take the supernatant; The precipitate was dried, and it was rice bran crude fiber, weighing 247.3g;

[0051] (3) Heat the supernatant of step (2) to 90°C, adjust the pH to 3.0 with citric acid reagent, stir continuously for 10 minutes, 3000r / min, 15 minutes, centrifuge to collect the...

Embodiment 3

[0061] A method for comprehensively utilizing rice bran, the method comprising the steps of:

[0062] (1) Weigh 1000g of rice bran, crush it through a 80-mesh sieve, use Soxhlet extraction method, use 95% n-hexane solvent to extract at 50°C for 2 hours, then filter the n-hexane solution, and dry the rice bran until the n-hexane is completely Volatility is defatted rice bran; the filtrate is completely evaporated with n-hexane with a rotary evaporator, and the rest is rice bran oil, weighing 110.2g;

[0063] (2) Pour the defatted rice bran in step (1) into 7L water, heat to 45°C, adjust the pH to 9.0 with NaOH reagent, stir continuously for 15min, centrifuge for 15min at a speed of 3000r / min, and take the supernatant; Precipitated and dried, it was rice bran crude fiber, weighing 537.6g;

[0064] (3) Heat the supernatant of step (2) to 90°C, adjust the pH to 3.0 with citric acid reagent, stir continuously for 10 minutes, 3000r / min, 15 minutes, centrifuge to collect the pre...

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Abstract

The invention discloses a method for comprehensively utilizing rice bran, which can be used for preparing four types of products, namely rice bran oil, rice bran crude fiber, rice bran egg white and rice bran peptide. A preparation process comprises the following steps of: extracting grease from rice bran serving as a raw material; separating rice bran crude fiber and rice bran egg white from therice bran by using an alkali-solution and acid-isolation method; adding protease into the rice bran egg white for hydrolyzing proteins; removing salt through anion and cation exchange resins by ultrafiltration fraction; and concentrating and drying to obtain rice bran biologically active peptide.

Description

technical field [0001] The invention relates to a method for comprehensively utilizing rice bran. Background technique [0002] Rice is the largest grain variety in my country. At present, the annual output is about 185 million tons, accounting for 42% of the country's total grain output. Paddy production accounts for 37% of the total food production in the world. In the process of processing rice into polished rice, the shell and the seed coat and embryo accounting for about 10% of the total weight must be removed. Rice bran is made from the seed coat and embryo and is the main by-product of rice processing. Research results and data at home and abroad show that rice bran is rich in various nutrients and physiologically active substances. Due to the different raw materials and processing techniques used in processing rice bran, the composition of rice bran is not exactly the same. Generally speaking, rice bran contains an average of 15% protein, 16%-22% fat, 3%-8% sugar...

Claims

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Application Information

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IPC IPC(8): C11B1/10C12P21/06C07K1/36A23L1/10A23L7/10
Inventor 陈燕飞亓培实何东平刘良忠何莉萍江思佳黄迪惠倪倩刘雪姣宋洋
Owner 武汉百信正源生物技术工程有限公司
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