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Tender stem callus plant regeneration induction culture medium for lonicera caerulea

A technology of indigo honeysuckle and induction medium, applied in plant cells and other directions, to achieve the effect of high probability and cost saving

Inactive Publication Date: 2012-02-29
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The object of the present invention is to solve the problems of low plant differentiation rate and high cost of culture medium in order to solve the existing tissue culture of Lonicera japonica mainly adopting axillary bud or shoot tip culture , the invention provides a kind of indigo honeysuckle young stem callus plant regeneration induction medium

Method used

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specific Embodiment approach 1

[0018] Specific embodiment 1: The present embodiment is the plant regeneration induction medium of the young stem callus of Lonicera japonica Lonicera, and the medium is composed of macroelements, trace elements and hormones, wherein the macroelements are composed of 900-920mg / L NH 4 NO 3 , 90~100mg / L of KH 2 PO 4 , 1000~1050mg / L of KNO 3 , 240~250mg / L of CaCl 2 2H 2 O, 200~210mg / L MgSO 4 ·7H 2 Composed of O and 15-18mg / L EDTA-Fe, the trace elements are composed of KI with a concentration of 0.83mg / L and MnSO with a concentration of 22.3mg / L 4 4H 2 O, 6.2 mg / L of H 3 BO 3 , 8.6mg / L ZnSO 4 ·7H 2 O, 0.025mg / L of Na 2 MoO 4 2H 2 O, 0.25mg / L CuSO 4 ·5H 2 O and 0.025mg / L of CoCl 2 ·6H 2 O, the hormone consists of 6-benzylaminopurine (6-BA) or kinetin (KT) at a concentration of 1.0-2.0 mg / L and indolebutyric acid (IBA) or naphthaleneacetic acid (NAA) at a concentration of 0.05-0.2 mg / L NAA) composition.

[0019] The young stem callus plant regeneration inductio...

specific Embodiment approach 2

[0022] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the macroelements are composed of NH with a concentration of 910 mg / L 4 NO 3 , 95mg / L of KH 2 PO 4 , 1025mg / L of KNO 3 , 245mg / L of CaCl 2 2H 2 O, 205mg / L of MgSO 4 ·7H 2 O and 16.5mg / L EDTA-Fe composition. Other parameters are the same as in the first embodiment.

[0023] The young stem callus plant regeneration induction medium (referred to as LD) of the present embodiment 1 Inorganic salt (macroelement and trace element) content in culture medium) is 2534.73mg / L, and the mass concentration ratio of inorganic salt content (4604.005mg / L) in existing MS culture medium is 1: 1.82.

[0024] The callus plant regeneration induction medium for young stems of Lonicera Lonicera Lonicerae in this embodiment reasonably reduces the content of inorganic salts in the medium, which is conducive to differentiation and cultivation, while high-concentration inorganic salts inhibit the regeneration of...

specific Embodiment approach 3

[0025] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that the hormone is composed of 6-benzylaminopurine (6-BA) with a concentration of 1.0-2.0 mg / L and 0.1-0.2 mg / L Indolebutyric acid (IBA) composition. Other parameters are the same as those in Embodiment 1 or Embodiment 2.

[0026] The young stem callus plant regeneration induction medium of Lonicera Lonicera in the present embodiment is used for culturing the young stems of Lonicera Lonicera, the callus induction rate can reach more than 90%, and the regeneration plant induction rate (i.e. plant differentiation rate) can reach 65%. %, saving 45.1% of the input cost compared with the control MS medium.

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Abstract

The invention relates to a tender stem callus plant regeneration induction culture medium for lonicera caerulea and solves the problems of low plant differentiation rate and high culture medium cost problem of the auxiliary bud or stem tip culture, which is the main tissue culture of lonicera caerulea. The culture medium consists of a large amount of macroelements, microelements and hormone, wherein the hormone consists of 6-benzyl aminopurine at a concentration of 1.0 to 2.0mg / L and indolebutyric acid or naphthylacetic acid at a concentration of 0.05 to 0.2mg. When the LD1 culture medium of the invention is used, the tender stems of lonicera caerulea can be easily induced to form calla, and the induction rates of the calla are all over 90 percent; and the probability of the induction of the calla into plants is high, the plant differentiation rate is up to 50 to 82 percent which is 66.6 to 173.3 percent higher than that of the conventional Murashige-Skoog (MS) culture medium, and thecost is saved by 45.1 percent compared with the reference MS culture medium.

Description

technical field [0001] The invention relates to a medium for inducing regeneration of callus plants of Lonicera japonica Lonicerae. [0002] Background technique [0003] Lonicera caerulea, also known as Lonicera caerulea, is commonly known as mountain eggplant, goat milk, black blind fruit, etc. It is a small perennial deciduous shrub. It is one of the unique small berries in Northeast Asia. Its distribution area is mainly in Russia and northern North Korea. , Japan and Northeast China's Greater and Lesser Xing'an Mountains, Changbai Mountains, Zhangguangcai Mountains and other forest areas. [0004] Indigo honeysuckle is another emerging small berry after black currant, raspberry, and lingonberry. It mainly grows in forest wetlands or forest margins, swamp meadows, and requires weakly acidic soil. The upper branches can withstand -40°C Above low temperature, strong adaptability. [0005] The honeysuckle fruit has high nutritional and health value, and is rich in carbohyd...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04
Inventor 赵恒田邓中枢黄福山沈云霞班文杰
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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