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System and method for rapidly detecting pesticide residues in fruits and vegetables on site

A technology for pesticide residues and detection systems, which is applied to the detection of the above-mentioned on-site rapid detection system and the field of fruit and vegetable pesticide residue rapid detection systems, can solve the problems of high price of tool enzymes and unrecoverable tool enzymes, and achieves elimination of pigment interference and easy extraction. , the effect of low cost

Inactive Publication Date: 2011-07-13
上海农信电子商务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the defect of the existing on-site detection methods for pesticide residues is that the tool enzymes used in the existing methods are expensive, and the tool enzymes cannot be recovered after use.

Method used

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  • System and method for rapidly detecting pesticide residues in fruits and vegetables on site
  • System and method for rapidly detecting pesticide residues in fruits and vegetables on site
  • System and method for rapidly detecting pesticide residues in fruits and vegetables on site

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Embodiment 1

[0023] The invention discloses a system and method for on-site rapid detection of pesticide residues in fruits and vegetables. The pesticides are detected by means of flow injection or direct immersion through an immobilized enzyme preparation process.

[0024] see figure 1 , in this embodiment, the present invention's on-site rapid detection system for pesticide residues in fruits and vegetables includes a server 10 and at least one sensor 20; the server is connected to each sensor to record the data detected by each sensor.

[0025] The sensor uses chicken liver esterase as a tool enzyme; the sensor extracts the chicken liver esterase, immobilizes the chicken liver esterase, and packs the chicken liver esterase; then detects the pesticides in fruits and vegetables; Treatment, and then treatment with alkali, acid and alkali to realize carrier recovery of the used enzyme column.

[0026] Preferably, the system includes a wireless router, and correspondingly, the server 10 and...

Embodiment 2

[0052] In this embodiment, the tool enzyme used in step A can also be other tool enzymes with its characteristics except chicken liver esterase.

Embodiment 3

[0054] The server is not included in this embodiment, but each sensor can also work independently. A display unit can be set on the sensor to display the detection result.

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Abstract

The invention discloses a system and method for rapidly detecting pesticide residues in fruits and vegetables on site. The system comprises at least a sensor, wherein the sensors adopt chicken liver esterase as tool enzyme; the sensors are used for extracting the chicken liver esterase, immobilizing the chicken liver esterase and packing the chicken liver esterase in a column; then the pesticides in the fruits and vegetables are detected with the enzyme column; and after detection, the enzyme column is firstly treated with buffer solution and then treated with acid base to realize carrier recovery of the used enzyme column. The system and the method have the following beneficial effects: the adopted detecting tool enzyme is the chicken liver esterase which has low cost and is easy to extract in a standard manner, so the cost is saved and the tool enzyme is easy to extract; the adopted reference column is homologous deactivated enzyme which is used for actually detecting the pesticides in the food and eliminates pigment interference, thus improving the detection precision; the absolute different value of the detection results does not exceed 6% of the arithmetic mean value; the coincidence rate of the positive results is 100%; the sample treatment time is 7-9 minutes; and the detection time is 9 minutes.

Description

technical field [0001] The invention belongs to the technical field of agricultural product quality and safety detection, and relates to an on-site detection system for pesticide residues, in particular to an on-site rapid detection system for pesticide residues in fruits and vegetables; meanwhile, the invention also relates to a detection method for the above-mentioned on-site rapid detection system. Background technique [0002] my country is the world's largest producer and user of pesticides, and the problem of pesticide residues in agricultural products is very serious. This not only poses a huge threat to human health and the ecological environment, but also greatly affects my country's food export trade. In particular, the "Positive List System" implemented by the Japanese government in May 2006 has caused my country's agricultural products to encounter the heaviest impact of foreign technical barriers since China's accession to the WTO, and nearly one-third of the ex...

Claims

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Application Information

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IPC IPC(8): C12Q1/44
Inventor 占锦川
Owner 上海农信电子商务有限公司