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Tobacco pollen medium as well as preparation method and application thereof

A technology of pollen medium and tobacco, applied in the direction of botany equipment and methods, applications, agricultural machinery and implements, etc., to achieve the effects of low cost, improved fertilization success rate, and good application prospects

Active Publication Date: 2011-07-20
YUNNAN ACAD OF TOBACCO AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no relevant research reports in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The flowers of Yunyan 87 (male parent) from the bud-bearing stage to the flowering stage were picked, the anthers were removed, and the anthers were removed and placed in a ventilated place indoors for 24 hours. The column heads were collected, dried at 30°C, ground and passed through a 60-mesh sieve, and sealed and stored at 4°C for later use. The prepared tobacco pollen medium and pure pollen of Yunyan 87 were evenly mixed at a weight ratio of 1:2, and used for pollination of MS Yunyan 87 (female parent).

Embodiment 2

[0019] Repeat Example 1 with the following differences: the stigma of Yunyan 85 (male parent) is collected, and the prepared tobacco pollen medium is applied to MS Yunyan 85 (female parent) for pollination.

Embodiment 3

[0021] Repeat Example 1 with the following differences: the stigma of K326 (male parent) is collected, and the prepared tobacco pollen medium is applied to pollination of MSK326 (female parent).

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Abstract

The invention discloses a tobacco pollen medium as well as a preparation method and application thereof. The preparation method of the tobacco pollen medium comprises the following steps of: picking flowers of a tobacco male parent from a bud containing period to an initial blooming period; removing anthers, placing on an indoor ventilating position, and cooling for 24 hours; collecting stigmas; drying under the condition of 30 DEG C; grinding, and then screening through a sieve of 60 meshes so as to obtain the tobacco pollen medium. The tobacco pollen medium and pure pollen are uniformly mixed according to the weight ratio of 1:2 and then used for the artificial pollination of tobaccos. The tobacco pollen medium can promote the germination of the pollen and the extension of pollen tubes, enhance the successful ratio of pollination, increase the output of tobacco seeds in unit area, reduce the usage amount of the pollen and effectively reduce the production cost of the tobacco seeds; and in addition, the invention changes wastes into valuables and has the advantages of easiness and convenience for operation, low cost and good application prospect.

Description

technical field [0001] The invention belongs to the technical field of tobacco hybrid seed production, and in particular relates to a tobacco pollen medium prepared by utilizing tobacco stigmas, and meanwhile, the invention also relates to a preparation method and application of the tobacco pollen medium. Background technique [0002] In recent years, the application of tobacco male sterile lines and hybrids in tobacco leaf production has become the leading direction of the world's tobacco agricultural industry. In the process of hybrid seed production, tobacco pollen is an extremely important production material. Due to the small amount of pollen in the male parent of tobacco and the difficulty in collecting it, the use of pure tobacco pollen for artificial pollination requires a large amount of pollen and a low effective utilization rate of pollen. Therefore, it is often necessary to plant a large number of male parents and invest a lot of manpower in collecting pollen to ...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01D91/04
Inventor 马文广郑昀晔李永平牛永志肖江海宋碧清索文龙邓盛斌
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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