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Method for cultivating earthworms for experiment

A breeding method and experimental technology, applied in animal husbandry and other directions, can solve the problems of poor parallelism, laboratory environment impact, complicated method operation, etc., and achieve the effect of maintaining quantity and activity, occupying small space and simple operation.

Inactive Publication Date: 2011-08-10
NANJING UNIV
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  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are not many studies on the breeding methods of experimental earthworms.
In the literature related to earthworm breeding methods, the breeding method introduced in "Technical Key Points of Earthworm Culture" ("Jiangxi Animal Husbandry and Veterinary Journal", 2004) is not strictly controlled, which may easily cause obvious individual differences among earthworms and poor parallelism, which will affect the experimental results. make an impact
In addition, in "Research on the Laboratory Feeding Method of Earthworms" ("Food Research and Development" Magazine, 2010), it introduced the method of feeding in boxes and baskets and making fermented feed at the same time. The method is complicated to operate, and the process of fermented feed will also affect the Influenced by the laboratory environment

Method used

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  • Method for cultivating earthworms for experiment
  • Method for cultivating earthworms for experiment
  • Method for cultivating earthworms for experiment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Breeding box: This embodiment uses a large finishing box. The height is 50 cm in length, 35 cm in width, and 35 cm in height. The lid has vent holes with a diameter of φ2. Earthworms dig and collect earthworms from the wild as earthworm species.

[0026] Breeding method: Spread a layer of sponge on the bottom of the culture box and spray tap water to moisten the surface; the depth of the breeding bed of E. vulgaris is 25 cm, and the depth of the breeding bed of E. vulgaris is 18 cm; the depth of the breeding bed is 18 cm; The aquaculture volume is controlled below 150-180, and the aquaculture volume of Eisenia vulgaris is controlled below 300-400. The breeding bed uses soil with a total organic carbon content of 2.5%. A mixture of cow dung and straw (with a ratio of 8: 2) was added at the initial stage of breeding. The initial input of the two types of earthworms was 0.5% and 1.0% of the soil weight, respectively. The positions of the bait were placed on the breeding bed...

Embodiment 2

[0028] The earthworms raised by this method were used for the earthworm survival rate experiment. The breeding conditions were the same as in Example 1, except that the survival rate of earthworms at different temperatures was tested, and the results can be seen image 3 .

Embodiment 3

[0030] The earthworms raised by this method were used in the study of the accumulation and degradation kinetics of pentachlorophenol in the body of the worms. Each jar contains 100 g of soil and 6 earthworms, each of which is about 2 g. Except that no bait was added, the other conditions were the same as in Example 1. The results showed that after 40 days, the experimental earthworms had the same activity as the newly collected earthworms in the field, and there was no significant change in body weight. The specific statistical results are shown in the attachment. Figure 4 And Figure 5 .

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Abstract

The invention discloses a method for cultivating earthworms for experiment, belonging to the technical field of cultivating special animals. The method comprises the following steps of: paving a layer of sponge in a cultivating box, spraying water until the sponge is wet; putting the sponge layer into a cultivating bed which is paved by soil and bait into layers according to different proportions, wherein the height of the cultivating bed is 18-25cm; when cultivating pheretima guillelmi, controlling pH of the soil within 6-7, placing the bait which accounts for 0.5-1% by weight of the soil at1-2cm of the surface of the soil, controlling water content of the cultivating bed within 50-70%, putting the earthworms in the cultivating bed; when cultivating eisenia foetida, controlling pH of the soil within 6-7, placing the bait which accounts for 1-2% by weight of the soil on the top of the soil, controlling water content of the cultivating bed within 40-80%, wherein the cultivation is carried out under the condition of illumination. The method is simple in operation, and capable of effectively improving survival and activity of the earthworms, and the conditions are easy to control.

Description

technical field [0001] The invention relates to a method for cultivating earthworms, in particular to a method for cultivating earthworms for experiments. Background technique [0002] Earthworm is a hermaphroditic, heterogeneous scavenging soil animal. It has the physiological characteristics of wide appetite, large food intake and the ability to secrete various enzymes in the body to decompose protein, fat, carbohydrates and cellulose. Organic waste is decomposed and transformed through the mechanical friction of the gizzard and the biochemical action in the intestinal tract. Earthworms are rich in edible and medicinal value. In the United States and Japan, refined earthworms have been used to bake biscuits, bread and meat substitutes (Xu Bin et al., 2010). The Chinese medicinal name of earthworms is Dilong, which can treat asthma, jaundice, typhoid fever and gum ulcers (Xu Bin et al., 2010). [0003] The test methods for acute toxicity of earthworms are included in the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
Inventor 季荣杨雪单军徐军郭红岩俞协治崔益斌
Owner NANJING UNIV
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