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Method for biologically testing chemoreception potential of plant root system secretions

A plant root system and bioassay technology, applied in botany equipment and methods, horticultural methods, horticulture, etc., can solve the problems of exudates affected by microorganisms, uneven distribution of root exudates, etc., and achieve the effect of eliminating competition

Inactive Publication Date: 2011-09-07
云南省农业科学院生物技术与种质资源研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] The technical problem to be solved in the present invention is to overcome existing (1) inhomogeneous distribution of root exudates in the agar co-cultivation method in the medium, (2) the interspecies competition between plants existing in the agar method and the delayed sowing method, the root system The technical problem that the exudates are affected by microorganisms in the environment, the purpose of the invention is to provide a simple, fast and accurate bioassay method for the evaluation of the allelopathic potential of root exudates of plant germplasm resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 Embodiment of the present invention

[0032] Test materials: rice and barnyardgrass, wherein rice is the donor plant, respectively (1) Kouketsumochi, which is a reported allelopathic variety; (2) Lemont, which is a reported non-allelopathic variety; (3) PI312777, (4) YAAS11002 is an African cultivated rice variety with strong competitiveness; (5) Yunlu 28 is an upland rice variety with strong competitiveness; (6) Purple Nuo, a local rice variety in Yunnan, has good stress resistance; (7) Huangke Nuo, a local rice variety in Yunnan; (8) Weixihonggu, a local rice variety in Yunnan, has strong cold tolerance; ( 9) Weixi Zigu, a local rice variety in Yunnan, has strong cold tolerance. The recipient (target) plant was barnyardgrass. The experiment was repeated 3 times.

[0033] The test steps of the embodiment of the present invention are as follows:

[0034] 1. Culture medium preparation: prepare an aqueous agar solution with a concentration of 0.3% in term...

Embodiment 2

[0043] Embodiment 2 is agar co-cultivation method

[0044] Agar co-cultivation method is reported by Australian scientists (Wu, H., D.Pratley, T., Memerle and T.Haig.2001.Screening methods for the evaluation of crop allelopathic potential.Bot.Rev.67(3):171- 180.), the specific steps are: 0.6% agar medium is sterilized and poured into a petri dish with a diameter of 9 cm, and the petri dish is divided into two equal parts with a sterilized thin cardboard, and 12 grains are sterilized on the surface 1. The paddy rice (donor plant) that germinates is divided into 3 rows, sows 3-4-5 seed on a part of petri dish with every row, at the same time in another part of this petri dish (that is another part separated by thin cardboard) with the same Sow 12 grains of barnyardgrass through surface disinfection and germination in the same way as above; do not sow rice, but sow 12 grains of barnyardgrass through surface disinfection and germination in the same way as above grass as a control...

Embodiment 3

[0049] Embodiment 3 delayed sowing method

[0050] Delayed Sowing Method by IRRI Scientists [3] The method reported is widely used in the evaluation of rice allelopathic potential and the determination of the growth inhibitory effect on weeds. The specific steps are: put a round filter paper at the bottom of the petri dish, then put it into a larger container, 3-5 Put the centimeter-wide long strip filter paper into the middle of the petri dish, and the long petri dish at both ends of the strip filter paper enters the container to become a filter paper bridge, and then put 50 ml of sterilized water into the larger container. 12 rice seeds (donor plants) that have been germinated and germinated are sown on both sides of the strip filter paper in 2 rows, and the entire culture container is placed in an incubator at 25-28°C for 7 days, and 15 germinated rice seeds are sown in the middle of the 2 rows of rice The germinated barnyardgrass seeds were not sown with rice and only sow...

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Abstract

The invention relates to a method for biologically testing chemoreception potential of plant root system secretions, belonging to the field of biological test technology.The method comprises the following steps: preparing agar liquid with a concentration less than or equal to 0.3% with water; sterilizing the agar liquid and pouring the agar liquid into a culture dish; disinfecting the donor plant and sowing the donor plant in the culture dish; sealing the culture dish, keeping the culture dish in a culture room with a temperature of 25-28 DEG C for 12 hours and culturing for 10-14 days in the bright / dark environment; removing all plants to be tested from the 11th day to the 15th day; disinfecting the receptor plant and sowing the receptor plant in the culture dish with the donor plant removed; sealing the culture dish, keeping the culture dish in a culture room with a temperature of 25-28 DEG C for 12 hours and culturing for 5-7 days in the bright / dark environment; then taking out the receptor plant to measure growth amount thereof and growth amount of the receptor plant not affected by the donor plant; calculating influence of the plant root system secretions on the growth of the receptor plant according to the measured growth amount to carry out chemoreception potential assessment.The invention is characterized by excluding competition among plant species for water and nutrient and fully reflecting effects the donor plant root system secretions have on the receptor plant.

Description

technical field [0001] The invention belongs to the technical field of bioassay, and in particular relates to a bioassay method for evaluating the allelopathic potential of plant root exudates. Background technique [0002] The phenomenon of mutual growth and mutual restraint among organisms has been recognized by humans since ancient times, but it was not officially named "allelopathy" by Austrian plant physiologist Hans Molisch until 1937, which is defined as the inhibition and promotion of biochemical interactions between all plants and microorganisms. In 1996, the International Allelopathy Society (International Allelopathy Society) made the latest definition: the function of individual organisms to affect the growth and development of other organisms around them by releasing compounds into the environment. Plant allelopathy is a chemical expression of plants' adaptation to the environment. The effect of plants on other plants through root exudates—"allelopathy" is a com...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G7/00
Inventor 郭怡卿金吉雄程在全孙一丁李维蛟殷富有钟巧芳付坚李定琴张敦宇王玲仙陈勇
Owner 云南省农业科学院生物技术与种质资源研究所
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