Cordyceps sinensis mycelium solid fermentation extract and application thereof to preparing anti-fatigue product

A kind of Cordyceps sinensis, solid fermentation technology, applied in the application of solid fermentation extract in anti-fatigue, Cordyceps sinensis (Sacc.) mycelium solid fermentation extract field, to achieve wide practicability and operability, good anti-fatigue effect, prolong Effect of Weighted Swim Time

Active Publication Date: 2011-09-07
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports or related technical patents on the use of Cordyce

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The collected wild Cordyceps sinensis (Cordyceps sinensis (Berk.) Sacc.) fruiting bodies were separated from tissues by conventional methods, and were connected to Cordyceps sinensis slant culture medium (according to its total mass fraction 100%, Consists of 1% glucose, 0.5% peptone, 0.3% malt extract powder, 0.3% yeast extract, 2% agar and the rest of the water) to obtain pure bacterial strains. Entity, tissue separation under aseptic conditions, inoculated on Cordyceps sinensis slant medium, placed at 15°C, and cultured in shading for 5-7 days. The starting strain was used as a control to compare the growth ability of the obtained strains, mainly to observe the growth of mycelia Speed, speed of color change and the number of primordia formed. Finally, the strains with better growth on the inclined plane were tested.) The strains were rejuvenated.

[0021] Insert the original bacterial strain into aseptic Cordyceps sinensis slant medium (according to its total mass fr...

Embodiment 2

[0029] The collected wild Cordyceps sinensis (Cordyceps sinensis (Berk.) Sacc.) fruiting bodies were separated from tissues by conventional methods, and were connected to Cordyceps sinensis slant culture medium (according to its total mass fraction 100%, 1.5% of glucose, 0.8% of peptone, 0.4% of malt extract powder, 0.4% of yeast extract, 2% of agar and the rest of the water) are cultivated to obtain pure bacterial strains. In order to prevent the degradation of bacterial strains, according to the conventional method of Example 1 Rejuvenate the bacteria.

[0030] Insert the original bacterial strain into the aseptic Cordyceps slant culture medium (according to its total mass fraction 100%, composed of glucose 1.5%, peptone 0.8%, malt extract powder 0.4%, yeast extract 0.4% by the amount of 0.3cm × 0.5cm size) %, agar 2% and the remainder of water), at 13°C, after dark culture for 33 days, light for 5 days, select the strains with fast color change and neat mycelium growth as the...

Embodiment 3

[0038] The collected wild Cordyceps sinensis (Cordyceps sinensis (Berk.) Sacc.) fruiting bodies were separated from tissues by conventional methods, and were connected to Cordyceps sinensis slant culture medium (according to its total mass fraction 100%, By 2% of glucose, 1% of peptone, 0.5% of malt extract powder, 0.5% of yeast extract, 2% of agar and the rest of the water), culture to obtain pure bacterial strain, in order to prevent the degradation of bacterial strain, according to the conventional method of Example 1 Bacteria are rejuvenated.

[0039] Insert the original bacterial strain into the aseptic Cordyceps slant culture medium (according to its total mass fraction 100%, composed of glucose 2%, peptone 1%, malt extract powder 0.5%, yeast extract 0.5% by the amount of 0.3cm × 0.5cm) %, agar 2% and the rest of the water), at 15°C, after 35 days of dark culture, and 6 days of light, select the bacterial classification that changes color quickly and the mycelium grows n...

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PUM

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Abstract

The invention relates to a cordyceps sinensis mycelium solid fermentation extract and application thereof to the aspect of preparing an anti-fatigue product. The cordyceps sinensis mycelium solid fermentation extract is prepared from wild cordyceps sinensis (Berk.) Sacc. fruiting bodies through the steps of strain collection, preservation and rejuvenation, preparation of mother strain, primary liquid strain and secondary production strain, preparation of a solid fermentation substance, extraction of the solid fermentation extract and the like. Compared with the conventional liquid fermentation method, a method for preparing the cordyceps sinensis mycelium solid fermentation extract has the characteristics of simple process, environmental friendliness, no waste liquid discharge, low cost and the like, so that the method has wide practicability and operability. Experiments prove that mice loaded swimming time can be obviously prolonged by the cordyceps sinensis mycelium solid fermentation extract prepared with the method, so that the extract has a good anti-fatigue effect and can be used for preparing the anti-fatigue product.

Description

technical field [0001] The invention relates to a fermented extract of Cordyceps sinensis mycelium, in particular to a solid fermented extract of Cordyceps sinensis (Berk.) Sacc. Applications in fatigue. Background technique [0002] In recent years, the research and development of anti-fatigue natural medicines has become a hot spot both at home and abroad. As a nourishing traditional Chinese medicine, Cordyceps sinensis has the effects of protecting the lungs and kidneys, relieving cough and reducing phlegm, treating impotence, spermatorrhea, waist and knee pain, etc. world. Modern research shows that Cordyceps sinensis has its unique advantages in eliminating exercise-induced fatigue and enhancing exercise ability. It can not only increase energy substances in athletes, but also regulate neuroendocrine, strengthen metabolism, etc., and stimulate the secretion and release of hormones in the body. Enhance the functions of the cardiovascular system, digestive system, hema...

Claims

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Application Information

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IPC IPC(8): C12N1/14A61K36/068A61P39/00
Inventor 李泰辉闫文娟劳景辉姜子德林群英宋斌沈亚恒李挺
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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