Cordyceps sinensis mycelium solid fermentation extract and application thereof to preparing anti-fatigue product
A kind of Cordyceps sinensis, solid fermentation technology, applied in the application of solid fermentation extract in anti-fatigue, Cordyceps sinensis (Sacc.) mycelium solid fermentation extract field, to achieve wide practicability and operability, good anti-fatigue effect, prolong Effect of Weighted Swim Time
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Embodiment 1
[0020] The collected wild Cordyceps sinensis (Cordyceps sinensis (Berk.) Sacc.) fruiting bodies were separated from tissues by conventional methods, and were connected to Cordyceps sinensis slant culture medium (according to its total mass fraction 100%, Consists of 1% glucose, 0.5% peptone, 0.3% malt extract powder, 0.3% yeast extract, 2% agar and the rest of the water) to obtain pure bacterial strains. Entity, tissue separation under aseptic conditions, inoculated on Cordyceps sinensis slant medium, placed at 15°C, and cultured in shading for 5-7 days. The starting strain was used as a control to compare the growth ability of the obtained strains, mainly to observe the growth of mycelia Speed, speed of color change and the number of primordia formed. Finally, the strains with better growth on the inclined plane were tested.) The strains were rejuvenated.
[0021] Insert the original bacterial strain into aseptic Cordyceps sinensis slant medium (according to its total mass fr...
Embodiment 2
[0029] The collected wild Cordyceps sinensis (Cordyceps sinensis (Berk.) Sacc.) fruiting bodies were separated from tissues by conventional methods, and were connected to Cordyceps sinensis slant culture medium (according to its total mass fraction 100%, 1.5% of glucose, 0.8% of peptone, 0.4% of malt extract powder, 0.4% of yeast extract, 2% of agar and the rest of the water) are cultivated to obtain pure bacterial strains. In order to prevent the degradation of bacterial strains, according to the conventional method of Example 1 Rejuvenate the bacteria.
[0030] Insert the original bacterial strain into the aseptic Cordyceps slant culture medium (according to its total mass fraction 100%, composed of glucose 1.5%, peptone 0.8%, malt extract powder 0.4%, yeast extract 0.4% by the amount of 0.3cm × 0.5cm size) %, agar 2% and the remainder of water), at 13°C, after dark culture for 33 days, light for 5 days, select the strains with fast color change and neat mycelium growth as the...
Embodiment 3
[0038] The collected wild Cordyceps sinensis (Cordyceps sinensis (Berk.) Sacc.) fruiting bodies were separated from tissues by conventional methods, and were connected to Cordyceps sinensis slant culture medium (according to its total mass fraction 100%, By 2% of glucose, 1% of peptone, 0.5% of malt extract powder, 0.5% of yeast extract, 2% of agar and the rest of the water), culture to obtain pure bacterial strain, in order to prevent the degradation of bacterial strain, according to the conventional method of Example 1 Bacteria are rejuvenated.
[0039] Insert the original bacterial strain into the aseptic Cordyceps slant culture medium (according to its total mass fraction 100%, composed of glucose 2%, peptone 1%, malt extract powder 0.5%, yeast extract 0.5% by the amount of 0.3cm × 0.5cm) %, agar 2% and the rest of the water), at 15°C, after 35 days of dark culture, and 6 days of light, select the bacterial classification that changes color quickly and the mycelium grows n...
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