Glass-micropipe-based single nanopore sensor and manufacturing method thereof

Abstract

The invention discloses a glass-micropipe-based single nanopore sensor which comprises a glass micropipe, a single nanopore, two fluid chambers, a first voltage source, two Pt electrodes, an ammeter and a second voltage source, wherein the glass micropipe is provided with a protuberance at one end; the single nanopore is arranged in the protuberance; the two fluid chambers are respectively arranged at both ends of the single nanopore and connected with each other through the single nanopore; the first voltage source is used for applying voltages to the two fluid chambers; the two Pt electrodes are arranged on the protuberance and arranged at the upside and downside of the single nanopore; the ammeter is used for detecting voltage between the two Pt electrodes and both ends of the ammeter are respectively connected with the two Pt electrodes; the second voltage source is used for applying voltage to the two Pt electrodes; and the single nanopore and the fluid chambers are all provided with electrolyte. The sensor provided by the invention uses four electrodes to obtain radial current and axial current, so that the sensor disclosed by the invention can have higher sensitivity. Meanwhile, the method disclosed by the invention is simple and can greatly reduce the manufacturing cost of the sensor.

Description

Technical field [0001] The invention relates to a device for detecting molecules and a manufacturing method thereof, and in particular to a single nanopore sensor based on glass microtubes and a manufacturing method thereof. Background technique [0002] Currently, nanochannels mainly include natural nanochannels and solid-state nanochannels. The preparation of solid nanopores is mainly carried out on solid thin film materials. The methods are as follows: ①Ion beam engraving, ion beam sputtering erodes the pore membrane material to make a nanopore or stimulates the material to transport and shrink into a nanopore; ②Micro-carving, first The master is made by photolithography technology, and then the metal is deposited on PDMS to prepare nanopores; ③Latent track etching, the polymer film is irradiated by high-energy heavy ions to produce latent tracks along the incident ion path, and then the potential ion orbits of the film Chemical etching is carried out to form a hole film; ④Hi...

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Problems solved by technology

However, current detection is to generate ion current by applying voltage in the axial direction, but applying voltage in the axial direction will limit its resolution and cannot judge the movement state of the measured medium in the hole.

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