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Method for synchronously measuring blood drug concentrations of multiple antidepressants

A technology of blood drug concentration and antidepressant, applied in the field of medical testing, can solve the problems of many interfering substances, unreliable retention time, unable to fully meet clinical requirements, etc., and achieve the effect of low analysis cost and fast and accurate method.

Inactive Publication Date: 2011-09-07
BEIJING GUGE MEDICINE DEV CO LTD
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, the sensitivity of the HPLC method cannot fully meet the clinical requirements for the detection of drugs at the nanogram level for the therapeutic window (such as the sertraline treatment concentration of the patient is usually 10-50ng ml -1 ), in addition, although the specificity of the HPLC method is better than that of the immunoassay method, the reliability of judging the substance only by the retention time is not reliable
Moreover, there are many interfering substances in biological samples, and it is not possible to monitor the concentration of antidepressant drugs well under the conditions of high sensitivity and high resolution.

Method used

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  • Method for synchronously measuring blood drug concentrations of multiple antidepressants
  • Method for synchronously measuring blood drug concentrations of multiple antidepressants
  • Method for synchronously measuring blood drug concentrations of multiple antidepressants

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Embodiment 1

[0030] Chromatographic conditions:

[0031] American Agilent RPLC 1200 high performance liquid chromatography, including online degassing, binary pump, column thermostat, automatic sampler, 6410Triple Quad LC / MS / MS triple quadrupole mass spectrometer and chromatography workstation; column: Zorbax SB -C18 (3.5μm, 3.0×150mm); mobile phase: methanol (containing 0.1% formic acid): water (containing 0.1% formic acid) = 66:34, flow rate 0.4mL min -1 , column temperature 25°C.

[0032] Sample pretreatment:

[0033] Take 200 μL of plasma sample, add 336ng·mL precisely -1 Promethazine solution (internal standard) 20 μL, vortexed for 10 s, then precisely added 600 μL of acetonitrile, vortexed for 30 s, centrifuged at 13000 rpm for 5 min, and 10 μL of the supernatant was injected.

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Abstract

The invention discloses a method for synchronously measuring blood drug concentrations of multiple antidepressants, wherein the method comprises the steps of: pre-processing a sample to be measured, analyzing the chromatographic column separation, and performing the detection by a mass detector. The method has the advantages that the samples are a few, the pre-processing procedure is simple, the analysis period is short, the detection sensitivity is high, the blood drug concentrations of a plurality of antidepressants can be detected synchronously, the application is wide, the cost is low, and the method is suitable for monitoring conventional blood drug concentrations.

Description

technical field [0001] The invention belongs to the field of medical testing, and relates to a method for analyzing and measuring drugs in vivo, in particular to a method for simultaneously measuring blood drug concentrations of multiple antidepressants. Background technique [0002] Depression (depression) is a common affective disorder mental disease, which has the characteristics of high incidence, high recurrence rate, high disability rate and high burden rate. [0003] Sertraline, Citalopram, Fluoxetine, Paroxetine, and Venlafaxine are traditional first-line antidepressant drugs. Due to the large individual differences in the pharmacokinetics of these drugs in the human body and the narrow therapeutic window, the interaction between drugs has a greater impact on the pharmacokinetics, and the pharmacokinetic characteristics are closely related to the antidepressant effect and the occurrence of adverse drug reactions. Therefore, it is of clinical significance to carry ou...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/72
Inventor 申屠建中窦静
Owner BEIJING GUGE MEDICINE DEV CO LTD
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