Method for monitoring drug resistance of spodostera exigua hubner to insecticides

A technology of beet armyworm and insecticide, which is applied in the fields of pesticide and entomology, can solve the problems of non-standard methods and complicated steps of monitoring methods, achieve uniform distribution, improve the effect of wetting and spreading, and improve the accuracy

Inactive Publication Date: 2011-09-07
SHANDONG AGRICULTURAL UNIVERSITY
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  • Abstract
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Problems solved by technology

[0004] The above monitoring work has indeed provided great help for the field control and resistance management of bee

Method used

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  • Method for monitoring drug resistance of spodostera exigua hubner to insecticides
  • Method for monitoring drug resistance of spodostera exigua hubner to insecticides
  • Method for monitoring drug resistance of spodostera exigua hubner to insecticides

Examples

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Embodiment 1

[0012] Example 1: The purpose of this experiment is to study the drug resistance level of emamectin benzoate in Tengzhou and Binzhou, Shandong.

[0013] In the field, samples were taken at five walking "W" points in Tengzhou and Binzhou, Shandong Province, and the larvae of beet armyworm above the third instar within a radius of 3 hectares were collected. The number of samples collected at each point was about 100 or more, and a total of about 500 larvae were raised indoors for one generation. The 3rd instar worms with uniform size were starved for 2 hours in a 24-well plate to determine the level of drug resistance. Accurately weigh 1.1111g of 90% emamectin benzoate technical substance, dissolve it with acetone and set the volume to a 100mL volumetric flask, and make 10000mg.L -1 Dilute the mother solution of emamectin benzoate to 2.00, 0.50, 0.13, 0.03, 0.01mg.L with 0.1% Tween-80 aqueous solution -1 For monitoring the resistance of beet armyworm Tengzhou population, dilute...

Embodiment 2

[0022] Example 2: The purpose of this experiment is to study the resistance level of tebufenozide in Tengzhou and Binzhou.

[0023] In the field, samples were taken at five walking "W"-shaped points in Tengzhou and Binzhou, and the larvae above the third instar of the beet armyworm within a radius of 3 hectares were collected. The number of samples collected at each point was about 100 or more, and a total of about 500 larvae were raised indoors for one generation. The 3rd instar worms with uniform size were starved for 2 hours in a 24-well plate and then used. Accurately weigh 1.0500g of 95% tebufenozide technical substance, dissolve it in acetone and make it into a 100mL volumetric flask to make 10000mg.L -1 Tebufenozide mother solution, diluted with 0.1% Tween-80 aqueous solution to 200.00, 100.00, 50.00, 25.00, 12.50mg.L -1 For monitoring the drug resistance of beet armyworm Tengzhou population, dilute the mother liquor with 0.1% Tween-80 aqueous solution to 400.00, 200.0...

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Abstract

The invention relates to a method for monitoring drug resistance of insecticides. The method comprises the following steps: (1) obtaining a species group of spodostera exigua hubner in at least one geographical region via field collection; (2) transferring the species group of spodostera exigua hubner collected in fields into indoor for propagating one generation; (3) hungering the propagated one generation three-age spodostera exigua hubner for 2 hours, and performing the drug resistance measurement; (4) diluting at least one insecticide crude drug into five concentrations that the death rate is 10%-90%, and dipping cabbage round leaves to obtain poisonous leaves which are used for feeding the spodostera exigua hubner; and (5) checking the result after a certain time to obtain the drug resistance level of the spodostera exigua hubner in a region to a certain insecticide. The method determines the resistance condition of the spodostera exigua hubner in fields to insecticides via monitoring the drug resistance of the species group of spodostera exigua hubner to insecticides, so that effective guidance can be supplied for the resistance management and integrated control of spodostera exigua hubner.

Description

(1) Technical field: [0001] The invention relates to a method for monitoring insecticide resistance of beet armyworm larvae, belonging to the fields of pesticide science and entomology. (two) background technology: [0002] Beet armyworm (Spodoptera exigua (Hübner)), commonly known as cabbage brown armyworm, belongs to Lepidoptera family Noctuidae, and is an important agricultural pest distributed worldwide. Because of its miscellaneous feeding habits and wide range of hosts, it is particularly fond of vegetables, soybeans, peanuts, corn and cotton, which poses a great threat to agricultural production. The outbreak of beet armyworm is mainly caused by the following reasons: first, the special climatic conditions of high temperature and drought are conducive to the occurrence of beet armyworm; second, the changes in planting structure in recent years, especially since the 1990s The continuous increase of the planting area provides sufficient food for the beet armyworm, and ...

Claims

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Application Information

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IPC IPC(8): G01N33/50
Inventor 慕卫周超
Owner SHANDONG AGRICULTURAL UNIVERSITY
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