Method for ungluing germ cells of takifugu obscurus

Abstract

The invention discloses a method for ungluing germ cells of takifugu obscures, and the method comprises the following steps: (1) placing the germ cells of takifugu obscures into a barrel-shaped container with a smooth inner wall, wherein the quantity of the placed germ cells is not more than 1 / 8 of the volume of the container; (2) slowly pouring an ungluing solution into the container, wherein the ratio of the ungluing solution to the germ cells is 4:1; (3) using a stirrer to clockwise stir the germ cells for 3-5 minutes, observing while stirring, stopping stirring when the ungluing solution is thickened, and removing the liquid on the upper layer; and (4) repeating the steps (2) and (3) for 2-3 times until the germ cells are completely unglued. The method provided by the invention has the advantages that the method for preparing the ungluing solution is simple and practicable, the ungluing effect is uniform and thorough, the degree of damages to the germ cells is reduced to the lowest, and the fineness and transparent degree of the walls of the germ cells are well ensured.

Description

technical field [0001] The invention relates to a method for debonding fertilized eggs of puffer pufferfish. Background technique [0002] Pufferfish (Fugu), commonly known as puffer fish, bang fish, Ba fish. Due to the deterioration of the ecological environment of puffer and the overfishing of puffer, the number of puffer has declined sharply in recent years. Therefore, people have carried out research on the artificial breeding technology of puffer obscurus and puffer chrysanthemum, and formed a set of perfect human reproduction technology. In the conventional artificial reproduction technology, most of the viscous fertilized eggs of Puffer obscurus are hatched by artificial insemination and artificial egg squeezing to obtain fertilized fish eggs, and the debonding of the viscous fertilized eggs has always been the most perfect method in the process of human reproduction of Puffer obscurus. subject. At present, people generally use mud or talcum powder solution as a deb...

AI Technical Summary

Problems solved by technology

At present, people generally use mud or talcum powder solution as a debonding agent for debonding, but these debonding methods often have insufficient uniformity of the debonding agent; uneven and incomplete debonding; Thick debonding material reduces the smoothness and transparency of the egg wall and affects the respiration and physiological metabolism of the fertilized eggs during the hatching process, resulting in a significant decrease in the hatching rate of the fertilized eggs of pufferfish obscurus and an increase in the rate of hatching deformities, which can be described as time-consuming, laborious and effective. bad