Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bacillus subtilis strain and application thereof

A Bacillus subtilis, strain technology, applied in bacteria, applications, microorganisms and other directions, can solve the problems of fermentation flavor, poor color and texture, difficult food industrial production, etc., and achieve good color and flavor, outstanding aroma, and stability. Good results

Inactive Publication Date: 2011-09-28
GUIZHOU UNIV
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pure-bred fermented tempeh has problems such as poor fermentation flavor, color and texture, and is difficult to apply to food industry production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0030] (1) Isolation and screening of Bacillus subtilis BJ3-2 strain

[0031] 1) Strain isolation and purification

[0032] Take 2g of commercially available bacteria-type Douchi samples in major areas such as Bijie and Zunyi in Guizhou, put them in a 18g triangle flask of sterile water, add 0.1% Tween 80, incubate at 180rpm for 30min, and then serially dilute to 10-2-10-9 , with 3 replicates for each dilution gradient. After standing for 10 minutes, take 1 mL of the liquid, evenly spread it on the beef extract peptone plate, and incubate at 37°C for 24 hours. Gram staining and spore staining, the single colonies of G+ bacillus were purified for 3 generations and kept in beef extract peptone slant test tubes, 4-5 strains were reserved for each sample, and the plate morphology of the strains was recorded and classified.

[0033] 2) Primary screening

[0034] The inoculated strains were placed in beef extract peptone liquid test tubes, cultivated at 30°C for 12h, inoculated i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a Bacillus subtilis strain and an application thereof. The bacterium is 0.5mu m in width and 2.6mu m in length and is Gram-positive; the shape of the spore is oval, the spore is in the center of the bacterium; the cysts fail to expand; the bacterium does not have parasporal crystal; and the bacterium has flagella. The bacterial colony is pure white and is irregular in shape; the center of the bacterial colony is raised in a concentric ring shape, the bacterial colony is non-transparent and has dry surface; and bacteria are slightly sticky and easy to pick up. The colorand luster of the fermented soybean fermented by the Bacillus subtilis strain are similar to that of the fermented soybean fermented naturally; and the strain can not generate gas, and the strain hashigher enzyme-producing capability and better fermentation flavor and is suitable for the industrial pure fermention production of fermented soybean.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a bacillus subtilis strain, and also relates to the application of the bacillus subtilis strain in realizing the pure fermentation of bacterial fermented soya bean. Background technique [0002] Bacterial tempeh is currently mainly produced by traditional natural fermentation, and pure fermenting has not been realized. As a result, the water tempeh produced by wet tempeh is often polluted by bacteria, gas production, capping and secondary pollution are serious, and toxins are easily produced. And the salt content is too high, these factors all seriously threaten the health of human body. [0003] However, the production of Japanese natto and Indonesian tianpei have already entered industrialized production due to the adoption of pure-bred fermentation technology, which has a large production and sales output. The main strains in the naturally fermented bacterial fermented soya bean ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20A23L1/20C12R1/125A23L11/00
Inventor 吴拥军
Owner GUIZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com