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Strain A56WU capable of raising anti-drought and anti-heat ability of leguminous trees and culture method of root nodule seedlings thereof

A culture method and strain technology, applied in the field of biological strain resources, can solve problems such as breakthrough progress has not been achieved, and achieve the effect of improving rapid growth and drought resistance.

Inactive Publication Date: 2011-10-05
SOUTHWEST FORESTRY UNIVERSITY
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  • Abstract
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AI Technical Summary

Problems solved by technology

However, so far, domestic research on this issue is still in its infancy, and breakthroughs have not yet been made.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: the bacterial strain of the present invention is after selecting in 2004 in Yuanmou, Yuanjiang and Dongchuan three hot and dry valleys in Yunnan Province to collect leguminous tree species with resistance to dryness and heat, such as the root nodules of Taiwan Acacia etc. Cultivation, through the symbiotic culture test of sterile seedlings of different leguminous species, after nodulation, the nodulation seedlings were subjected to dryness and heat resistance tests, and the selection of energy The excellent strains that can obviously promote tree growth and dry heat resistance, and have a significant difference from the control biomass are selected after molecular identification. The strain is characterized by strong resistance to dryness and heat, and has the ability to form nodules on multiple tree species. After successful inoculation, it can improve the resistance to dryness and heat of the host. The whole experimental process has been carried out in S...

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Abstract

The invention relates to a biological strain resource, in particular to a strain capable of raising fast-growing, anti-drought and anti-heat ability of leguminous trees in dry-hot valley areas, and a culture method of root nodule seedlings thereof. Strain A56 WU disclosed in the invention is Bradyrhizobium eikanii, CGMCC No: 4591. The culture method comprises isolation and purification, microscopic examination, back grafting and inoculation of the strain. The strain disclosed in the invention is a good strain selected from the strains in dry-hot valley areas and capable of effectively raising fast-growing, anti-drought ability of leguminous trees in dry-hot valley areas, so that the strain has important utilization value for vegetation restoration of dry-hot valley areas, allowing leguminous trees to live through the crisis securely under the threat of dry-hot environment, and ensuring the survival rate of large area afforestation in dry-hot valley areas.

Description

technical field [0001] The invention relates to a biological strain resource, in particular to a strain capable of effectively improving the rapid growth of leguminous tree species in dry-hot valley areas and having excellent stress resistance, that is, resistance to dryness and heat, and a method for cultivating the nodulated seedlings thereof. Background technique [0002] In nature, there is a common symbiotic system in which root nodule bacteria and legumes form root nodules and fix nitrogen in the air for plant nutrition, which can increase the source of nitrogen in the soil. Improve the host's absorption of phosphorus, potassium and other nutrients and water, therefore, enhance the stress resistance and disease resistance of the host plant, increase the yield and variety of the host, and promote the growth and development of the plant. [0003] Leguminous tree species have high economic value, are highly resistant to adverse environments, and have significant advantage...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01G1/00C12R1/01
Inventor 马焕成伍建榕曾小红周利平张东华郭瑞超
Owner SOUTHWEST FORESTRY UNIVERSITY
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