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Culture medium for culturing lilium pumilum tissues

A technology of tissue culture and lily lily, applied in the field of plant biology, can solve the problems of limited reproduction number, disease infection quality, degradation, etc.

Active Publication Date: 2011-10-12
INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The artificial propagation of Lily lily still mainly adopts the method of separate planting small bulbs, sowing and scale cuttings, the number of reproduction is limited and it is easy to cause disease infection and quality degradation
However, there are few studies on tissue culture propagation that can be used to solve the problem of degeneration and rejuvenation. Therefore, it has become one of the urgent tasks for scientific researchers to carry out in-depth research on tissue culture of Lily lily.

Method used

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  • Culture medium for culturing lilium pumilum tissues
  • Culture medium for culturing lilium pumilum tissues
  • Culture medium for culturing lilium pumilum tissues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1. Materials and screening statistical methods of lily

[0017] The thin-leaf lily used in the experiment was collected from Daqingshan, a suburb of Hohhot, and the bulbs collected were planted in the company's experimental nursery. According to the progress of the experiment, samples were taken at different times and supplemented at any time. The explant is the bulb of lily.

[0018] Use MS as the basic medium, 3.5g / L carrageenan as the curing agent, 3% edible white sugar instead of sucrose as the carbon source, tap water instead of distilled water, and add different ratios of cytokinin and auxin for the medium. Of screening.

Embodiment 2

[0019] Example 2. Selection of the best culture medium for each stage of tissue culture of Lilium tenuiflora

[0020] 1. Selection of differentiation medium

[0021] Set the concentration of 6-BA to 0.2mg / L, 0.5mg / L, 1.0mg / L, and 2.0mg / L. The concentration of NAA is set to 0.1mg / L, 0.2mg / L, 0.3mg / L, Four concentration gradients of 0.5 mg / L were used in different combinations. One bulb was connected to each bottle during inoculation, and the occurrence of adventitious buds on different media was observed.

[0022] It can be seen from Table 1 that the 7 hormone combinations can induce lily scales to differentiate into small bulbs, but the differentiation rate of each combination is different. MS+6-BA 0.1mg / L+NAA 0.1mg / L is the thin-leaf lily scale medium It is the best induction medium. It not only has a high induction rate, but also has a large number of new buds per explant and the highest differentiation rate, which can reach 60.0%.

[0023] The specific statistical formula is:

[00...

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Abstract

The invention relates to a culture medium for culturing lilium pumilum tissues. The best culture mediums for culturing the lilium pumilum tissues at different stages are screened; the proportion of various hormone in the culture medium is determined; and the culture mediums prepared according to the formula can meet the nutrient requirement and growth and development of the lilium pumilum at eachstage. The culture mediums comprise a first generation induction culture mediums consisting of MS, 1.0 mg / L of 6-BA and 0.1 mg / L of NAA, a subgeneration proliferation culture medium consisting of MS,0.2 mg / L of 6-BA and 0.01 mg / L of NAA, a rooting culture medium consisting of 1 / 2 MS and 0.5 mg / L of IBA and a capitulum culture medium consisting of 1 / 2 MS and 0.5 mg / L of IBA and 2 percent of sugar.

Description

Technical field [0001] The invention belongs to the field of plant biotechnology, and specifically relates to a culture medium at various stages in the tissue culture of Lilium pumilum. The present invention screens out the best culture medium for different periods of lily thin-leaf tissue culture, and determines the proportion of each hormone in the medium. The medium prepared according to the formula of the present invention can satisfy the nutrition of lily thin-leaf Needs and growth. Background technique [0002] Lilium pumilum, also known as Shandan, is a perennial bulbous flower in the lily family. The lily lily grows in arid and semi-arid areas. It has strong vitality and can survive the winter smoothly in the wild. It can also grow normally under the condition of little rain and no watering. It can meet the greening requirements in terms of performance and beauty. Use and promote it in the drought-resistant greening landscape. However, due to threats from both humans a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王召明梁德霖姚裕琪
Owner INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD
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