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Bacillus mucilaginosus and applications thereof

A technology of jelly-like spores and bacilli, applied in the field of microorganisms, can solve the problems of difficult removal of heavy metal ions, unstable effective silicon content, and impact on soil quality

Active Publication Date: 2012-11-28
领先生物农业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the silicon fertilizer applied in the field is mainly industrial waste residue containing silicate, such as iron and steel smelting slag, copper smelting slag, aluminum smelting red mud, fly ash silicon calcium fertilizer and yellow phosphorus slag, cement kiln dust and silicon magnesium potassium fertilizer, etc. , the effective silicon content of these fertilizers is unstable, and the heavy metal ions in them are difficult to remove
Therefore, long-term application of these fertilizers will have a serious impact on soil quality.
[0004] The soil contains a lot of silicon, and the weight percentage of silicon dioxide is 20%-80%, but most of it is in crystals and cannot be absorbed and utilized by plants

Method used

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  • Bacillus mucilaginosus and applications thereof
  • Bacillus mucilaginosus and applications thereof
  • Bacillus mucilaginosus and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] Embodiment 1: jelly-like bacillus CGMCC NO.4667 culture

[0096] (1) Strain activation:

[0097] Prepare a slant medium containing the following ingredients: sucrose 10g / l, dipotassium hydrogen phosphate 0.5g / l, sodium chloride 0.2g / l, silicon dioxide 1g / l, magnesium sulfate 0.2g / l, calcium carbonate 1g / l l. Yeast extract 0.4g / l, agar powder 18g / l, pH 7.2;

[0098] A ring of jelly-like Bacillus CGMCC NO.4667 was picked on the slant medium, and the inoculated slant was placed in a constant temperature incubator at a temperature of 28° C. for 48 hours.

[0099] (2) Seed cultivation:

[0100] Prepare a seed medium containing the following ingredients: starch 5g / l, dipotassium hydrogen phosphate 3g / l, ammonium sulfate 1g / l, yeast extract 0.5g / l, sucrose 3g / l, magnesium sulfate 2g / l, calcium carbonate 1g / l, ferric chloride 0.02g / l, silicon dioxide 1g / l, pH7.2;

[0101] Take step (1) and inoculate one ring of activated jelly-like Bacillus CGMCC NO.4667 in the seed cultur...

Embodiment 2

[0105] Example 2 Mutagenesis of jelly-like bacillus CGMCC NO.4667 and plate primary screening

[0106] (1) Prepare the bacterial suspension of the type bacterial strain:

[0107] Pick out a single colony of the starting strain ACCC 10013, insert it into a simple medium (yeast extract powder 5g / l, peptone 10g / l, sodium chloride 5g / l, pH7.2-7.4), and place it on a constant temperature shaker at 32°C and 180rpm Available after 18 hours of cultivation. Take 5ml of the cultured bacterial solution and put it into a conical flask with glass beads and 45ml of sterile water, shake it at a speed of 180rpm for 20min to make a uniform bacterial suspension and dilute it to 10 -4 Backup.

[0108] (2) Ultraviolet mutagenesis and breeding of strains with strong desilicon performance

[0109] Add the bacterial suspension prepared in step (1) to the plate, the thickness of the bacterial liquid is 0.2cm, let it irradiate for 5min at a distance of 30cm from a 20-watt ultraviolet lamp, and carr...

Embodiment 3

[0115] Example 3 Mutagenesis of jelly-like bacillus CGMCC NO.4667 and plate primary screening

[0116] (1) Prepare the bacterial suspension of the type bacterial strain:

[0117] Pick out a single colony of the starting strain (ACCC 10013), insert it into a simple medium (yeast extract powder 5g / l, peptone 10g / l, sodium chloride 5g / l, pH7.2), and place it on a constant temperature shaker at 32°C and 180rpm Available after 16-20 hours of cultivation. Take 5ml of the cultured bacterial solution and put it into a conical flask with glass beads and 45ml of sterile water, shake it at a speed of 180rpm for 20min to make a uniform bacterial suspension and dilute it to 10 -4 Backup.

[0118] (2) Ultraviolet mutagenesis and breeding of strains with strong desilicon performance

[0119] Add the bacterial suspension prepared in step (1) to the plate, the thickness of the bacterial liquid is 0.2cm, let it irradiate for 5min at a distance of 30cm from a 20-watt ultraviolet lamp, and car...

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Abstract

The invention relates to a bacillus mucilaginosus strain which is obtained through ultraviolet mutagenesis and has higher Si-releasing capability, and the CGMCC ((China General Microbiological Culture Collection Center) NO of the strain is 4667. The invention also relates to applications of the strain. Experiments prove that, by adopting the strain, the available silicon content and water-solublesilicon content of soil can be increased by more than 20%; the root growth can be promoted, the dry weight of the root system can be increased by more than 30%; the tiller number of rice can be increased by more than 20%; the yield of rice can be increased by more than 10%; and the total nitrogen content of the harvested rice plant can be increased by 7.4%, the total phosphorus content can be increased by 7.41%, the total potassium content can be increased by 3.72%, and the total silicon content can be increased by 7.89%.

Description

【Technical field】 [0001] The invention belongs to the technical field of microorganisms. More specifically, the present invention relates to a jelly-like bacillus, and also relates to the use of the jelly-like bacillus as a biological silicon fertilizer. 【Background technique】 [0002] Silicon is listed as the fourth largest element fertilizer after nitrogen, phosphorus and potassium in the international soil community. It is mainly used in silicon-loving crops such as rice, wheat, and corn, especially rice is the most sensitive to silicon. Rice is known as a silicic acid plant. Applying silicon fertilizer on silicon-deficient soil can increase the number of rice tillers, increase the number of effectively harvested spikes, increase the rate of seed setting, promote the accumulation of dry matter and increase yield, and improve rice quality. In addition, silicon fertilizer has the most significant effect on rice blast resistance, which is mainly manifested in increasing th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/07
Inventor 杜迎辉庄钟娟孙杉杉徐志文苏海榆张财源朱瑞艳
Owner 领先生物农业股份有限公司
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