Nucleus inserting method for nuclear-free pearl grain breeding
A technology of non-nucleated pearls and nucleation method, which is applied in fish farming, application, climate change adaptation, etc., to achieve the effects of high efficiency, solving the problem of the source of edible pearl raw materials, and simple nucleation method
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Embodiment 1
[0013] (1) Take 500g of seawater pearl and crush it into 3-20 mesh coarse particles (the pearl core is fragile and crushed into finer particles. The coarse powder is passed through a 30 mesh sieve to obtain coarse particles without sieving. The composition is pearl with a certain degree of toughness), remove the particles attached to the pearl nucleus, and the remaining coarse particles are 43g of pure pearl. The pearl is crushed with a high-speed pulverizer and passed through a 120-mesh sieve to obtain 120-mesh pearl powder. Continue to smash after 120 meshes, until 120 meshes are passed. The obtained pearl powder 42g (loss of 1g) was sterilized with steam for later use; 42g medical gelatin was used and 21kg of clean sterile water was used to prepare a 0.2% gelatin mixture, which was heated and sterilized, and 42g of sterilized pearl powder was added, stirred evenly, and prepared It becomes a mixture containing 0.2% pearl powder.
[0014] (2) Choose 1,000 healthy pearl oysters,...
Embodiment 2
[0017] (1) Take 800g of seawater pearl and crush it into 3-20 mesh coarse particles, remove the particles with the pearl nucleus, and the remaining coarse particles are pure pearl 75g, crush the pearl with a high-speed crusher and pass through a 120 mesh sieve , Get 120 mesh pearl powder, continue to pulverize before 120 meshes, until 120 meshes are passed. The obtained pearl powder 73g (loss of 2g) was sterilized with steam for later use; 16g hyaluronic acid and 4kg clean sterile water were used to prepare a 0.4% hyaluronic acid mixture, heat sterilized, add 45g sterilized pearl powder, and stir Evenly, formulated into a 1.8% mixture containing pearl powder.
[0018] (2) Choose 500 healthy pearl oysters, open the pearl oysters according to the method of inserting beads, and use the METTLER EDP3 electric multi-channel pipette to fill in the mixed liquid containing 1.2% pearl powder, 5ml each time, multi-channel transfer The liquid container contains 8 dispensing tubes. One end o...
Embodiment 3
[0021] (1) Take 1000g of seawater pearls and crush them into 3-20 mesh coarse particles, remove the particles with pearl nucleus, and the remaining coarse particles are 96g pure pearls, crush the pearls with a high-speed crusher and pass through a 120 mesh sieve , Get 120 mesh pearl powder, continue to pulverize before 120 meshes, until 120 meshes are passed. The obtained pearl powder 95g (loss of 1g) was sterilized with steam for later use; 9g medical gelatin was used and 3kg of clean sterile water were used to prepare a 0.3% gelatin mixture, heated and sterilized, and 95g of the sterilized pearl powder was added, stirred evenly, and prepared It becomes a mixture containing 3.2% pearl powder.
[0022] (2) Choose 500 healthy pearl oysters, open the pearl oysters according to the method of inserting beads, and use the METTLER EDP3 electric multi-channel pipette to fill in 3.2% pearl powder-containing mixture, each time 5ml, multi-channel transfer The liquid container contains 8 d...
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