Method for extracting total flavonoids from sambucus chinensis
A technology of total flavonoids and extracts, which can be used in pharmaceutical formulations, non-central analgesics, medical preparations containing active ingredients, etc., can solve the problems of low product purity, achieve good quality, high purity, and reduce dosage.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0014] Grind the whole herb of Lu Ying, take 3kg of medicinal material, divide it into three equal parts, put them in three extraction tanks respectively, take the three extraction tanks as a group, add 5 times the amount of 80% ethanol solution as a solvent, and extract twice, each The extraction time is 3 hours, the extract is obtained by filtration, the extract is concentrated under reduced pressure to obtain a concentrate, the concentrate is separated by AB-8 macroporous resin, first washed with distilled water to remove impurities, and then sequentially washed with 60% and 85% ethanol Solution elution, the eluent rich in flavonoids was concentrated under reduced pressure to no alcohol smell, left to settle, and the precipitate was refluxing with 99% ethanol to dissolve and recrystallize twice, and dried to obtain 49.6g of total flavonoids. was 65.7%.
Embodiment 2
[0016] Grind the whole herb of Lu Ying, take 3kg of medicinal materials, divide them into three equal parts, and place them in 3 extraction tanks respectively, with 3 extraction tanks as a group, add 3 times the amount of 85% ethanol solution as a solvent, extract 3 times, each The extraction time is 2 hours, the extract is obtained by filtration, and the extract is concentrated under reduced pressure to obtain a concentrated solution. The concentrated solution is separated by LSA-40 macroporous resin. Solution elution, the eluent rich in flavonoids was concentrated under reduced pressure to no alcohol smell, left to settle, and the precipitate was refluxing with 90% ethanol to dissolve and recrystallize twice, and dried to obtain 43.8g of total flavonoids. was 68.2%.
Embodiment 3
[0018] Grind the whole herb of Lu Ying, take 4kg medicinal material, divide it into four equal parts, put them in 4 extraction tanks respectively, take 4 extraction tanks as a group, add 4 times the amount of 75% ethanol solution as a solvent, extract twice, each time The extraction time is 1.5h, the extract is obtained by filtration, the extract is concentrated under reduced pressure to obtain a concentrate, the concentrate is separated by SPD300 macroporous resin, the impurities are first washed with distilled water, and then 60% and 80% ethanol solution For elution, the flavonoid-rich eluent was concentrated under reduced pressure until it had no alcohol smell, and then left to settle, and the precipitate was refluxing with 90% ethanol to dissolve and recrystallize once, and then dried to obtain 50.6 g of total flavonoids with a content of 69.0%.
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More