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Coaxially encapsulated cell suspensions and microtissues

A technology of cell suspension and microtissue, applied in the field of regenerative medicine

Inactive Publication Date: 2011-11-09
KONINK PHILIPS ELECTRONICS NV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Second, the supply of heterogeneous (micro)tissues, such as vascular or neural tissue, remains a major challenge

Method used

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  • Coaxially encapsulated cell suspensions and microtissues
  • Coaxially encapsulated cell suspensions and microtissues
  • Coaxially encapsulated cell suspensions and microtissues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0074] According to another embodiment of the present invention, the porous membrane comprises a poly(meth)acrylic material produced by polymerization of at least one (meth)acrylic monomer and at least one polyfunctional (meth)acrylic monomer.

[0075] According to another embodiment of the present invention, the (meth)acrylic monomer is selected from the group comprising: (meth)acrylamide, (meth)acrylic acid, hydroxyethyl (meth)acrylate, (meth)acrylic acid Ethoxyethoxyethyl ester, hydroxyethyl methacrylate, isobornyl (meth)acrylate, isobornyl methacrylate or mixtures thereof.

[0076] According to a further embodiment of the present invention, the multifunctional (meth)acrylic monomer is bis-(meth)acryloyl and / or tri-(meth)acryloyl and / or tetra-(meth)acryloyl and and / or penta-(meth)acryloyl monomers.

[0077] According to another embodiment of the present invention, the multifunctional (meth)acrylic monomer is selected from the group comprising bis(meth)acrylamide, tripropyl...

Embodiment 1

[0172] Eukaryotic epidermoid carcinoma cells A431 (ATCC# CRL-1555) were cultured in medium (Dulbecco's Modified Eagle's Medium; DMEM, Gibco) under standard cell culture conditions (37°C, 5% CO2, ~95% humidity) , the medium was supplemented with 10% fetal calf serum (FCS), 1% penicillin / streptomycin and 1% Glutamax (Invitrogen).

[0173] After two days, the cells formed a confluent layer and the medium was removed. The cells were washed twice with phosphate-buffered saline (PBS), then incubated with EDTA / trypsin for 10 minutes. Add 10 ml of culture medium to generate a cell suspension.

[0174] Cell suspension (1×10 5 cells / ml) were fed into the central cylinder of the coaxial extruder, while the separation materials [acrylamide (10 w / w%), biscarylamide (biscarylamide) (0.5 w / w% and photoinitiator irgacure 2959 (0.1 %)) in water] is fed into the outer cylinder.

[0175] The monomer solution in the outer cylinder and the cell suspension in the inner cylinder were coextruded...

Embodiment 2

[0179] Example 1 was repeated using the pulsed extrusion mode with the shutter in front of the UV source continuously open. The flow distribution used is as follows:

[0180] At t = 0, the monomer solution in the outer cylinder was extruded at a flow rate of 1 m / min, while the flow rate of the cell suspension was 0 m / min.

[0181] At t = 0.5 s, the flow velocity in the outer cylinder was decreased to 0.5 m / min, while the flow in the inner cylinder (cell suspension) was increased from 0 to 0.5 m / min.

[0182] At t = 1.5 s, the flow of the cell suspension was stopped again, while the flow in the outer cylinder was simultaneously restored to 1 m / min.

[0183] At t = 2 seconds, all flows were set to 0.

[0184] The experimental setup resulted in the formation of closed-ended compartments containing the A431 cell suspension and encapsulated by a surrounding polyacrylamide layer.

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Abstract

The present invention provides for the coaxial encapsulation of a plurality of cells in a single elongated compartment. By this encapsulation, the cells are protected by at least one layer of separation material and kept in close contact, which leads to a better vitality of the encapsulated cells and consequently results in higher chances to form microtissue. Methods and devices for the production of such encapsulated cell compartments are disclosed as well as medical uses of such compartments in cell, tissue therapy and tissue engineering.

Description

field of invention [0001] The present invention relates to the field of regenerative medicine, in particular to cell therapy and tissue engineering, and provides devices and methods for coaxial encapsulation of cell suspensions and / or production of microtissues. Also, coaxially encapsulated cell suspensions, microtissues and tissues, and their medical applications are disclosed. Background technique [0002] Regenerative medicine is an emerging discipline in the field of medical science. There are numerous methods and approaches used in regenerative medicine. [0003] Cell therapy is based on the delivery of cells, especially stem cells, to damaged tissue areas in order to restore tissue function. [0004] In tissue engineering, scaffolds and cells are used to grow tissue outside the body. The engineered tissue is then implanted in the patient to replace damaged or lost tissue. [0005] Cell handling and growth are key factors in these applications. Typically, the cells...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00A61L27/38B01J13/02A61K35/12
CPCA61K2035/126A61L27/38B01J13/04A61L27/58
Inventor R.库尔特E.彼得斯R.彭特曼D.J.布罗尔D.哈尔特
Owner KONINK PHILIPS ELECTRONICS NV
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