Extraction method for envelope protein of grouper iridovirus

Abstract

The invention relates to an extraction method for envelope protein of grouper iridovirus. According to the method, virus particles are purified by sucrose density gradient centrifugation; purified viruses are treated by 1% sodium dodecyl sulfonate (SDS); and virus envelope proteins are separated. The invention is mainly directed at virus envelope proteins, is favorable for promoting development of research on virus envelope proteomics and for further research on functions of grouper iridovirus envelope proteins in the process of virus infecting of hosts and for understanding of the interacting mechanism between viruses and hosts at molecular and cellular level, and provides a convincing scientific basis and application basis for controlling iridovirus diseases and developing viral vaccines.

Description

technical field [0001] The invention relates to a method for extracting a virus envelope protein, in particular to a method for extracting a grouper iridescent virus envelope protein. Background technique [0002] Viral structural proteins are proteins essential for viral morphogenesis and are essential components for the formation of infectious mature virus particles. In enveloped viruses, the structural proteins of the virus mainly include capsid protein, envelope protein and matrix protein. Among these proteins, the envelope protein has received extensive attention because it is located on the outermost side of the virus particle and is considered to be a ligand molecule for virus invasion adsorption and recognition. The envelope is a layer of membrane that covers the viral capsid. The components of the viral envelope mainly come from the host cell and are composed of proteins, lipids, and polysaccharides. After the virus is assembled and released from the host cell, th...

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