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Extraction method for envelope protein of grouper iridovirus

An extraction method, iridescent virus technology, applied in the field of virus envelope protein extraction, can solve the problems of lack of comparison and research, and achieve the effect of promoting research progress

Active Publication Date: 2011-11-16
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is a lack of comprehensive comparison and research on the envelope extraction methods of enveloped iridescent viruses.

Method used

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  • Extraction method for envelope protein of grouper iridovirus
  • Extraction method for envelope protein of grouper iridovirus
  • Extraction method for envelope protein of grouper iridovirus

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Embodiment Construction

[0027] The present invention will be further described below in conjunction with accompanying drawings and examples.

[0028] ,Material

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Abstract

The invention relates to an extraction method for envelope protein of grouper iridovirus. According to the method, virus particles are purified by sucrose density gradient centrifugation; purified viruses are treated by 1% sodium dodecyl sulfonate (SDS); and virus envelope proteins are separated. The invention is mainly directed at virus envelope proteins, is favorable for promoting development of research on virus envelope proteomics and for further research on functions of grouper iridovirus envelope proteins in the process of virus infecting of hosts and for understanding of the interacting mechanism between viruses and hosts at molecular and cellular level, and provides a convincing scientific basis and application basis for controlling iridovirus diseases and developing viral vaccines.

Description

technical field [0001] The invention relates to a method for extracting a virus envelope protein, in particular to a method for extracting a grouper iridescent virus envelope protein. Background technique [0002] Viral structural proteins are proteins essential for viral morphogenesis and are essential components for the formation of infectious mature virus particles. In enveloped viruses, the structural proteins of the virus mainly include capsid protein, envelope protein and matrix protein. Among these proteins, the envelope protein has received extensive attention because it is located on the outermost side of the virus particle and is considered to be a ligand molecule for virus invasion adsorption and recognition. The envelope is a layer of membrane that covers the viral capsid. The components of the viral envelope mainly come from the host cell and are composed of proteins, lipids, and polysaccharides. After the virus is assembled and released from the host cell, th...

Claims

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Application Information

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IPC IPC(8): C12P21/02C07K14/01C07K1/14C12R1/93
Inventor 秦启伟欧阳征亮公杰黄晓红黄友华
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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