Tissue culture method for populus yunnanensis Dode with tender stem as explant

A technique of tissue culture and explants, applied in the field of plant tissue culture, can solve the problems of high requirements for exogenous hormones, large amount of petioles, single materials, etc., and achieve the effect of high germination rate and convenient and quick materials
CN102246700AInactive Publication Date: 2011-11-23SOUTHWEST FORESTRY UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SOUTHWEST FORESTRY UNIVERSITY
Publication Date
2011-11-23
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a tissue culture method for populus yunnanensis Dode with a tender stem as an explant, belonging to the tissue culture method for plants. The tissue culture method comprises the following steps of: acquiring populus yunnanensis Dode branches growing for three years; carrying out water planting and then taking the tender stem as the explant; flatly placing an aseptic tender stem in a differential medium; growing fasciculate adventitious buds on callus; and respectively connecting into a multiplying and rooting culture medium, transplanting a domestication matrix, and the like. According to the tissue culture method disclosed by the invention, materials can be conveniently and rapidly obtained, culture media and domestication matrixes at various phases are respectively screened and compared; and the invention provides a method for industrial rapid reproduction and seedling culture of the populus yunnanensis Dode as well as the research for physiologic genetic characteristic of the populus yunnanensis.
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Description

technical field

[0001] The invention belongs to plant tissue culture method. Background technique

[0002] Yunnan poplar ( Populus yunnanensis ) is a tree species of Populus genus Populus in Salicaceae. There are still a small number of original wild plants of Populus yunnanensis only in some high-altitude mountainous areas. Extremely rare. As a model plant in forest tree genetics and breeding, the tissue culture of Populus yunnanensis includes basic medium and exogenous hormone selection, explant selection, disinfection, differentiation, proliferation and rooting culture, hardening and transplanting and other steps. In the selection of poplar explants, the axillary buds, leaves, and shoot tips connected to the shoot apex meristem have strong morphogenetic ability, and the corresponding tissue parts are usually selected from excellent trees before rejuvenation and then the explants are taken. Jin Shunyu et al. used leaves as explants in "Tissue Culture and Plant Regenerat...

Claims

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