Tissue culture method for populus yunnanensis Dode with tender stem as explant

A technique of tissue culture and explants, applied in the field of plant tissue culture, can solve the problems of high requirements for exogenous hormones, large amount of petioles, single materials, etc., and achieve the effect of high germination rate and convenient and quick materials

Inactive Publication Date: 2011-11-23
SOUTHWEST FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method is used as a laboratory research, and under industrial conditions, the amount of petiole material is large, and the requirements for basal medium and exogenous hormones are relatively high. possible combinations of media for comparison

Method used

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  • Tissue culture method for populus yunnanensis Dode with tender stem as explant
  • Tissue culture method for populus yunnanensis Dode with tender stem as explant
  • Tissue culture method for populus yunnanensis Dode with tender stem as explant

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Embodiment Construction

[0029] (1) Disinfection of explants

[0030] Three-year-old Populus yunnanensis branches that grow robustly and are free from diseases and insect pests were taken from the campus of Southwest Forestry University. After all the leaves on the branches were removed, they were placed in hydroponics at room temperature for one month. After the tender stems grew out, they were cut off as explant materials. Rinse the tender stems in a beaker with tap water for 2 hours, put them into a triangular flask, add 2% sodium hypochlorite, shake for 8 minutes, take out the tender stem material, rinse the petiole with sterile water for 3 times; then disinfect the tender stems with 70% ethanol 40sec, then put into 4 sterile bottles respectively, Ⅰ: +2% sodium hypochlorite for 10min; Ⅱ: +10%H 2 o 2 10min; Ⅲ: +0.1%HgCl 2 7min; IV: +0.1%HgCl 2 7min+2% sodium hypochlorite for 8min. Rinse the petiole 6 times with sterile water. MS was selected as the basal medium for inoculation. The MS formula...

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Abstract

The invention discloses a tissue culture method for populus yunnanensis Dode with a tender stem as an explant, belonging to the tissue culture method for plants. The tissue culture method comprises the following steps of: acquiring populus yunnanensis Dode branches growing for three years; carrying out water planting and then taking the tender stem as the explant; flatly placing an aseptic tender stem in a differential medium; growing fasciculate adventitious buds on callus; and respectively connecting into a multiplying and rooting culture medium, transplanting a domestication matrix, and the like. According to the tissue culture method disclosed by the invention, materials can be conveniently and rapidly obtained, culture media and domestication matrixes at various phases are respectively screened and compared; and the invention provides a method for industrial rapid reproduction and seedling culture of the populus yunnanensis Dode as well as the research for physiologic genetic characteristic of the populus yunnanensis.

Description

technical field [0001] The invention belongs to plant tissue culture method. Background technique [0002] Yunnan poplar ( Populus yunnanensis ) is a tree species of Populus genus Populus in Salicaceae. There are still a small number of original wild plants of Populus yunnanensis only in some high-altitude mountainous areas. Extremely rare. As a model plant in forest tree genetics and breeding, the tissue culture of Populus yunnanensis includes basic medium and exogenous hormone selection, explant selection, disinfection, differentiation, proliferation and rooting culture, hardening and transplanting and other steps. In the selection of poplar explants, the axillary buds, leaves, and shoot tips connected to the shoot apex meristem have strong morphogenetic ability, and the corresponding tissue parts are usually selected from excellent trees before rejuvenation and then the explants are taken. Jin Shunyu et al. used leaves as explants in "Tissue Culture and Plant Regenerat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 辛培尧胥辉段安安李根前周军何承忠刘岩
Owner SOUTHWEST FORESTRY UNIVERSITY
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