Lung-kidney deficiency type lung cancer mouse model

A mouse model, the technology of lung and kidney deficiency, applied in medical science, veterinary instruments, animal husbandry, etc., can solve the problem of unreported animal models of lung and kidney deficiency lung cancer

Inactive Publication Date: 2011-11-23
LONGHUA HOSPITAL SHANGHAI UNIV OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology

The establishment of an animal model of lung cancer with both lung and kidney deficiency has far-reaching significance for the research on the mechanism of anti-lung cancer effect of traditional Chinese medicine. Unfortunately, there is no report on the animal model of lung cancer with both lung and kidney deficiency

Method used

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  • Lung-kidney deficiency type lung cancer mouse model
  • Lung-kidney deficiency type lung cancer mouse model
  • Lung-kidney deficiency type lung cancer mouse model

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Embodiment Construction

[0017] The present invention will be further described below in conjunction with embodiment.

[0018] (1) Building a model

[0019] Twenty-four inbred C57BL / 6 mice were randomly divided into normal group, Lewis lung cancer cell subcutaneous xenograft tumor model group (model group for short), and Lewis lung cancer cell xenograft tumor group with lung and kidney deficiency type (composite model group for short). Group of 8.

[0020] ① Establishment of Lewis lung cancer cell xenograft model: the concentration of Lewis lung cancer cell line was adjusted to 1×10 7 / ml; use a 1ml syringe to take 0.2ml of the above suspension (containing 2×10 cells) 6 ), and injected subcutaneously in the left axilla of the mouse to complete the mouse tumor inoculation.

[0021] ② Establishment of the composite model group: The mouse model of lung qi deficiency and kidney deficiency and the Lewis lung cancer cell xenograft model were used to jointly establish a model. Lung qi deficiency model wa...

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Abstract

The invention belongs to the technical field of Chinese medicine lung cancer syndrome and relates to a building method for a lung-kidney deficiency type lung cancer mouse model. The lung-kidney deficiency type lung cancer mouse model can be used for preparing an animal model of lung-kidney deficiency type lung cancer disease combination. In the model, a preparation method that a mouse lung Qi deficiency and kidney deficiency model and a Lewis lung cancer cell transplantation tumor model are used for joint molding is adopted, i.e., a Lewis lung cancer cell is subjected to subcutaneous injection to build a lung cancer transplantation tumor model, a smoked stimulation method is adopted for building a lung Qi deficiency model, and hydrocortisone is subjected to intraperitoneal injection to build a lung deficiency model. The model is simple to operate, has the advantages of short molding period, high success rate and good repeatability and stability, and withstands the measurement and verification by a prescription (medicine). Most importantly, the model conforms to the pathologic features of lung cancer and has the characteristics of the Chinese medicine theory. The Chinese medicine theoretical knowledge can be combined to comprehensively analyze the scientificity of the mouse lung-kidney deficiency type lung cancer animal model, thus a scientific research tool is provided for researching the lung cancer resistance of the traditional Chinese medicine.

Description

technical field [0001] The invention belongs to the technical field of lung cancer syndrome research in traditional Chinese medicine, and in particular relates to a mouse model of lung and kidney deficiency type lung cancer, which can be used for the preparation and evaluation of lung cancer lung and kidney deficiency type lung cancer disease-syndrome combined animal models. Background technique [0002] Lung cancer is one of the most common malignant tumors that seriously endanger human life and health, and its morbidity and mortality rank first among malignant tumors. In recent years, the research on how to prevent and treat lung cancer has become the focus of international academic circles, and it is also an important research content in the research field of "population and health", which is urgently needed and needs to be developed in my country. Traditional Chinese medicine treatment of advanced lung cancer has the characteristics of safety and few side effects, which ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D7/00A01K67/027
Inventor 王菊勇郭净王青郑展
Owner LONGHUA HOSPITAL SHANGHAI UNIV OF TRADITIONAL CHINESE MEDICINE
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