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Peptide capable of transporting DNA to target neurocytes and brain

A nerve cell and targeting technology, which is applied in the field of biological science and drug carrier, can solve the problem of inability to target DNA transportation, and achieve the effect of low size and better biocompatibility

Inactive Publication Date: 2011-11-23
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, there has been no example of using a peptide carrier to efficiently carry DNA into animal neurons for expression. In addition, the peptide carrier itself has no selectivity for cells or tissues, and cannot deliver DNA to target cells. Specific site, which also largely limits its application in biotherapeutic research

Method used

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  • Peptide capable of transporting DNA to target neurocytes and brain
  • Peptide capable of transporting DNA to target neurocytes and brain
  • Peptide capable of transporting DNA to target neurocytes and brain

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Embodiment Construction

[0025] The peptide of the present invention consists of a targeting sequence, a linking sequence and a DNA binding sequence. The targeting sequence is the acetylcholine receptor recognition ligand, the DNA binding sequence is 9 polyarginine, and the amino acid sequence of the peptide is YTIWMPENPRPGTPCDIFTNSRGKRASNGGGGGrRrRrRrRr (abbreviated as RVG29-9rR ), where YTIWMPENPRGTPCDIFTNSRGKRASNG is the acetylcholine receptor recognition ligand, also known as the targeting peptide RVG29, which plays the role of enrichment into the target cell and its uptake, GGGG is the linker sequence, which plays the role of linking and eliminating steric hindrance, and rRrRrRrRr is 9-polyarginine, r is unnatural arginine, which plays the role of wrapping plasmid DNA.

[0026] RVG29-9rR uses the cations of the side chains in the 9-polyarginine sequence to combine with the anions on the DNA surface and compress them. The specific operation method is to combine the plasmid DNA to be carried with a c...

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Abstract

The invention discloses a peptide capable of transporting a DNA to target neurocytes and brain and belongs to the field of biological science and medicine carriers. The peptide is formed by combining a target sequence, a connecting sequence and a DNA combining sequence, wherein the target sequence is an acetylcholine receptor identifying ligand; and the DNA combining sequence is 9-poly-L-arginine, and the amino acid sequence of the 9-poly-L-arginine is represented by SEQ ID No.1 in a sequence list. The peptide can transport the DNA to the target neurocytes and brain.

Description

technical field [0001] The invention belongs to the field of biological science and drug carrier. Background technique [0002] Gene therapy is one of the most likely solutions to some brain diseases, but the blood-brain barrier prevents some drug molecules, including therapeutic genes, from entering the brain. Therefore, the development of a carrier that can transport genes across the blood-brain barrier to the brain is very important for the treatment of some brain diseases. Viral vectors have been shown to efficiently deliver genes to neurons and cells within the brain of living animals. However, viral vectors also have a series of disadvantages, such as complex construction and use of genes that can only carry small molecular weights, easy to cause inflammatory reactions, and potential safety issues, etc., which will affect their application in humans. Therefore, researchers have developed a series of artificially synthesized non-viral vectors that have looser require...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/00A61K47/42
Inventor 张玉慧骆清铭龚铖
Owner HUAZHONG UNIV OF SCI & TECH
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