Method for spinning silk from cocoon of silkworm expressing foreign gene, and product produced by employing the method

A transgenic, raw silk technology, applied in silk fiber production, reeling, surgical instruments, etc., can solve problems such as silk dye affinity and fabric texture damage

Inactive Publication Date: 2011-11-23
NAT INST OF AGROBIOLOGICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a result, the dye affinity and f

Method used

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  • Method for spinning silk from cocoon of silkworm expressing foreign gene, and product produced by employing the method
  • Method for spinning silk from cocoon of silkworm expressing foreign gene, and product produced by employing the method
  • Method for spinning silk from cocoon of silkworm expressing foreign gene, and product produced by employing the method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0139] [Example 1] Silk production using silkworm cocoons expressing foreign genes in silk glands

[0140] 1. Method

[0141] 1.1 Drying steps

[0142] Typically, the drying process gradually reduces the temperature from a maximum temperature of 115°C to 120°C to 60°C after about 5.5 hours have elapsed. This would give a dry percentage of about 42%. However, in this example, instead of using a temperature gradient, the cocoons were placed in a drier maintained at a constant temperature of 60°C and dried within 20 to 24 hours. As a result, the desired drying percentage ( figure 1 ).

[0143] 1.2 Cocoon cooking steps

[0144] In the present invention, in order to swell and soften cocoon layer sericin under low temperature (60° C. or lower) conditions, cocoon cooking is performed using alkaline agents, surfactants, enzymes, and the like. Examples using alkaline agents and surfactants are given below.

[0145] First, a solution of 0.1% to 0.4% sodium carbonate and 0....

Embodiment 2

[0153] [Example 2] Using fluorescent transgenic cocoon silk as interior accessories material

[0154] Fluorescent transgenic cocoon silk is used as a material for interior accessories. The fluorescent transgenic cocoon silk is directly glued and wound around the spherical mold by using the sericin protein carried by the filament itself. Remove from mold when dry. image 3 Interior fittings made by such methods are shown in . exist image 3 , the object on the left has no light source within it (external light only), the object in the middle uses a white light as its light source, and the object on the right uses a black light as its light source. Fluorescent emission due to black light was observed for internal parts made of fluorescent transgenic cocoon silk as the base material.

Embodiment 3

[0155] [Example 3] Application of cell-adhesive cocoons to base materials for artificial blood vessels

[0156] Using transgenic cocoon silk with cell-adhesive properties to generate the base material for artificial blood vessels ( Figure 4 ). Figure 4 The artificial blood vessel shown in has an internal diameter of 1.5mm to 5mm, and the base material is made of raw silk obtained by cocoon drying at 60°C, cocoon boiling at 60°C or below, and silk reeling at 50°C. Figure 5 It shows the artificial blood vessel material made of transgenic cocoon silk and PTFE ( The state when the artificial blood vessel material made of type resin) was transplanted into rats. Figure 6 The state 3 months after transplantation of these materials into rats is shown. Progression of cell growth was observed in the case of artificial vascular material made from transgenic cocoon silk.

[0157] [Reference example] Production of transgenic silkworm cocoons

[0158] To generate fluorescent trans...

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Abstract

A cocoon is dried under low temperature (60 DEG C or lower) conditions, rather than the conditions where the temperature is higher than 100 DEG C which has been employed conventionally for the production of a raw silk from a cocoon. Further, the boiling of a cocoon is carried out by employing a vacuum infiltration treatment at a low temperature (a subatmospheric pressure treatment), rather than a high-temperature treatment at 100 DEG C or higher or a treatment with hot water having a temperature around the boiling point which has been employed conventionally. As an alternate cocoon boiling method, a combination of the swelling of sericin with an alkali and the penetration of hot water into a cocoon layer by using a surfactant, an enzyme or the like is carried out. The silk reeling is carried out at a lower temperature than that has been employed conventionally. As a result, it is found that a raw silk can be produced from a cocoon of a silkworm that expresses a foreign gene without deteriorating the properties of a foreign protein contained in the cocoon of the silkworm.

Description

field of invention [0001] The present invention relates to a method for producing silk from the cocoons of silkworms expressing foreign genes and products obtainable by this method. More specifically, the present invention relates to a technique for cocoon drying, cocoon cooking, and silk reeling without causing loss of properties of the foreign gene-expressing cocoon in the step of drawing silk from the foreign gene-expressing cocoon. Background of the invention [0002] If the silkworm chrysalis in the living cocoon is allowed to be free, it will metamorphose into a silk moth, squeeze through the cocoon silk of the cocoon shell made of cocoon silk, and come out of the cocoon shell as a silkworm moth. Since this would make the cocoon impossible to reel, the pupae must be killed and the cocoon dried before the moth leaves the cocoon. Typically, cocoons are dried by setting the initial temperature to 115°C or higher (in some cases to 130°C or higher), and then gradually redu...

Claims

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Application Information

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IPC IPC(8): D01B7/00A01K67/033A61L17/00A61L27/00D01B7/04D01F9/18
CPCA01K2227/703A01K2267/01A01K2217/05D01B7/04
Inventor 高林千幸田村俊树町井博明饭塚哲也瀬筒秀树立松谦一郎木下晴夫宫崎荣子山田胜成栗原宏征朝仓哲郎桑原伸夫山口纯次中村敬吉井圭宫胁敦史唐泽智司青木里步
Owner NAT INST OF AGROBIOLOGICAL SCI
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