Propolis injection for preventing duck plague and preparation method thereof

A technology for injection and propolis, which is applied to medical preparations containing active ingredients, pharmaceutical formulations, and resistance to vector-borne diseases, etc., can solve the problems of low protection rate, many use times, economic losses, etc. Effect

Active Publication Date: 2011-11-30
山东施得维特生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Duck plague is an acute infectious disease caused by a virus. It involves a wide range of areas and has a high incidence rate. The mortality rate is sometimes above 90%. Great economic loss. At the same time, due to improper handling of dead ducks, the spread of the virus often results in greater consequences.
At

Method used

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  • Propolis injection for preventing duck plague and preparation method thereof

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Comparison scheme
Effect test

Embodiment 1

[0021] Under aseptic conditions, take the internal organs (including heart, liver, spleen, lung, brain) of ducks that died of duck plague and put them into a tissue masher to mash, and take 10% of the total weight of the mashed duck tissues , 90% of the total weight, mixed with 0.8% normal saline to form a mixed tissue suspension, and put it in a centrifuge at 4 000 rpm for 20 minutes, take the supernatant, and put it on the After adding 2 000 units of penicillin and 2 000 units of streptomycin per milliliter of supernatant to the supernatant, duck plague seed virus was formed. Put the duck plague seed virus into 0.8% normal saline and dilute the volume by 100 times to form a duck plague seed poison dilution, and inoculate the duck embryo allantois of 9 days old with 0.1 ml of the duck plague seed poison dilution , after inoculation, choose the dead duck embryos in 48 hours of hemorrhage, edema and other characteristics, put them into a tissue masher and mash them to get duck ...

Embodiment 2

[0023] Under aseptic conditions, take the internal organs (including heart, liver, spleen, lung, brain) of ducks that died of duck plague and put them into a tissue masher to mash them, and take 15% of the mashed duck tissues of the total weight , accounting for 85% of the total weight, mixed with 0.8% normal saline to form a mixed tissue suspension, and put it into a centrifuge and centrifuge at 4000 rpm for 30 minutes, take its supernatant, and put it in the supernatant After adding 2 000 units of penicillin and 2 000 units of streptomycin to each milliliter of supernatant, duck plague seed virus was formed. Put the duck plague seed virus into the normal saline with a concentration of 0.8% and dilute it 100 times in volume to form a duck plague seed poison dilution, inoculate the duck embryo allantois of 11 days old with the duck plague dilution of 0.1 ml dose, and inoculate Select the dead duck embryos of features such as hemorrhage and edema in 80 hours and put them into a...

Embodiment 3

[0025] Under aseptic conditions, take the internal organs (including heart, liver, spleen, lung, brain) of ducks that died of duck plague and put them into a tissue masher to mash, and take 20% of the total weight of mashed duck tissues , accounting for 80% of the total weight, mixed with 0.8% normal saline to form a mixed tissue suspension, and put it into a centrifuge and centrifuge it at 4 000 rpm for 20 minutes, take its supernatant, and put it on the After adding 2000 units of penicillin and 2000 units of streptomycin to each milliliter of supernatant in the supernatant, duck plague seed virus will be formed. Put the duck plague seed virus into 0.8% normal saline and dilute it 100 times in volume to form a duck plague seed virus dilution, and inoculate 12-day-old duck embryo urine with 0.1 ml of the duck plague seed virus dilution. In the capsule, after inoculation, select the duck embryos that died in 48 hours of hemorrhage, edema and other characteristics and put them i...

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Abstract

The invention relates to the field of animal preventive medicine, in particular to a method for preparing injection for preventing animal epidemic diseases. The bee glue injection for preventing duck plague consists of bee glue aqueous solution, tissue homogenate of duck embryos which are dead after being inoculated and infected with duck plague virus, and inactivation liquid. The preparation method comprises the step of adding the inactivation liquid into mixed liquid of the duck embryo tissue homogenate and bee glue to obtain the bee glue injection for preventing the duck plague. The bee glue injection for preventing the duck plague, which is prepared by the method, has the obvious effect on the prevention of the duck plague, the protective rate is improved to 90 to 100 percent, the protective period is prolonged to 6 to 12 months, action producing time is shortened to 5 days, and the expected effect can be achieved only by one-time injection.

Description

technical field [0001] The invention relates to the field of animal preventive medicine, in particular to a method for preparing an injection for preventing animal epidemics. Background technique [0002] Duck plague is an acute infectious disease caused by a virus. It involves a wide range of areas and has a high incidence rate. The mortality rate is sometimes above 90%, which brings great economic losses to the breeding unit or individual. At the same time, due to the treatment of dead ducks Improper use often causes the spread of the virus and produces even greater consequences. At present, the problems of the main vaccines for preventing duck plague are short immunization period (generally about 3 months), high frequency of use, long time to produce effect (14 days), and low protection rate (50-80%). Therefore, The harm brought to the poultry breeding industry is serious and causes economic losses. Contents of the invention [0003] One of the objects of the present ...

Claims

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Application Information

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IPC IPC(8): A61K35/64A61K39/245A61P31/22A61K35/28A61K35/30A61K35/34A61K35/407A61K35/42A61K35/48A61K35/57A61K35/644
CPCY02A50/30
Inventor 杨金龙粟剑彭祥伟付利芝殷素会
Owner 山东施得维特生物工程有限公司
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