Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of freeze-drying protective agent, preparation method and application thereof

A drying protective agent and drying technology, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve problems such as development lag, long-term storage of finished products, and transportation restrictions

Active Publication Date: 2011-11-30
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
View PDF2 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the development of traditional protective agents for biological products is relatively lagging behind, which limits the long-term storage and transportation of finished products

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The components of the freeze-drying protective agent are as shown in formula 1 in table 4,

[0040] Preparation method following steps:

[0041] 1) Mix the components according to the above formula 1;

[0042] 2) Sterilize the above-mentioned freeze-drying protective agent at a temperature of 100° C. for 30 minutes.

[0043] Applying the prepared freeze-drying protective agent to Lactobacillus has a better protective effect on Lactobacillus during freeze-drying.

[0044] Preparation of Lactobacillus starter with the above-mentioned freeze-drying protective agent: first, add the Lactobacillus bulgaricus enriched by centrifugation to the above-mentioned freeze-drying protective agent in a mass ratio of 1:1, and mix the two evenly in a sterile state . Then the prepared Lactobacillus bulgaricus proliferation medium was stored in an environment of 4°C for 12 hours.

[0045] The Lactobacillus bulgaricus freeze-dried starter produced by the above-mentioned freeze-drying pr...

Embodiment 2

[0049] The composition of freeze-drying protective agent is as shown in formula 2 in table 4,

[0050] Preparation method following steps:

[0051]1) Mix the components according to the above formula 2;

[0052] 2) Sterilize the above-mentioned freeze-drying protective agent at a temperature of 100° C. for 30 minutes.

[0053] Applying the prepared freeze-drying protective agent to Lactobacillus has a better protective effect on Lactobacillus during freeze-drying.

[0054] Preparation of Lactobacillus starter with the above-mentioned freeze-drying protective agent: first, add the Lactobacillus bulgaricus enriched by centrifugation to the above-mentioned freeze-drying protective agent in a mass ratio of 1:1, and mix the two evenly in a sterile state . Then the prepared Lactobacillus bulgaricus proliferation medium was stored in an environment of 4°C for 12 hours.

[0055] The Lactobacillus bulgaricus freeze-dried starter produced by the above-mentioned freeze-drying protect...

Embodiment 3

[0059] The composition of freeze-drying protective agent is as shown in formula 3 in table 4,

[0060] Preparation method following steps:

[0061] 1) Mix the components according to the above formula 3;

[0062] 2) Sterilize the above-mentioned freeze-drying protective agent at a temperature of 100° C. for 30 minutes.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a freeze drying protective agent. Based on 100 parts, the freeze drying protective agent is prepared from the following components in parts by weight: 12 parts-20 parts of degreased milk powder, 0.5 part-3 parts of sodium glutamate, 0.1 part-0.5 part of sodium ascorbate, 0.5 part-3 parts of glycerol, 0.05 part-0.3 part of cystine, 10 parts-20 parts of trehalose and the balance of water. The invention provides a method for preparing the freeze drying protective agent. The method comprises the following steps: mixing the components; and sintering for 30 minutes at the temperature of 100 DEG C so as to prepare the freeze drying protective agent. The freeze drying protective agent is applied to freeze drying of microorganisms, especially lactobacillus, thereby exerting the protective function of the freeze drying protective agent to the greatest extent and reducing the death of thallus in the freeze drying process so as to greatly improve the survival rate of thallus.

Description

technical field [0001] The invention relates to a freeze-drying protective agent, in particular, the present invention relates to a lactobacillus freeze-drying protective agent, a preparation method and an application thereof. Background technique [0002] Freeze-drying is a drying process in which wet materials are frozen into a solid state at a lower temperature, and then the solid moisture is directly sublimated into a gaseous state under high vacuum to remove it, also known as sublimation drying. In the process of freeze-drying, the two processes of freezing and drying will cause damage, death and passivation of certain enzyme protein molecules of some microbial cells. If the protective agent is not properly selected in the freeze-drying process, the fermentation activity will decrease. [0003] Lactobacillus is one of the important strain resources of microorganisms in the dairy industry. It has good fermentation properties and can improve or regulate the balance of in...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12R1/225
Inventor 刘先龄南康小红生庆海刘卫星
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com