Bioflocculant fermentation method with mycelium pellet as vector

A technology of fermented organisms and mycelium balls, which is applied in the field of fermented biological flocculants, can solve the problems of being unsuitable for large-scale industrial fermentation production, low production efficiency, and high production costs, so as to reduce the chance of bacterial contamination, improve production efficiency, and flocculate The effect of rate stabilization

Inactive Publication Date: 2011-11-30
HARBIN INST OF TECH
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  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of high energy consumption, low production efficiency, high production cost and unsuitability for large-scale industrial fermentation production in the fermentation method of the existing biological flocculant, and provides a fermentation method using mycelium balls as a carrier. The Bioflocculant Method

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  • Bioflocculant fermentation method with mycelium pellet as vector
  • Bioflocculant fermentation method with mycelium pellet as vector
  • Bioflocculant fermentation method with mycelium pellet as vector

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specific Embodiment approach 1

[0009] Specific embodiment 1: The method of fermenting biological flocculant with mycelium pellets in this embodiment is carried out in the following steps: 1. Rhizobium radiata and Bacillus sphaericus are mixed in a volume ratio of 1:1 and then inoculated into 100 mL of flocculant. Culture culture medium in a 250mL Erlenmeyer flask, placed in a shaker with a temperature of 20~30℃ and a rotating speed of 120~160r / min for 18~30 hours to obtain a seed solution of flocculent fungus; 2. The spore suspension of Aspergillus niger Inoculate into a 250 mL Erlenmeyer flask containing 100 mL of mycelial pellet culture medium, and place it in a shaker at a temperature of 28-32°C and a rotation speed of 140r / min for 1-7 days to obtain mature hyphae; The mycelium pellets were rinsed with sterilized phosphate solution and added to a 250 mL Erlenmeyer flask containing 100 mL of flocculent culture medium, and then added the flocculent seed liquid at a volume ratio of 1% to 10%, and placed at a ...

specific Embodiment approach 2

[0015] Specific embodiment two: this embodiment is different from specific embodiment one in that in step one, it is placed in a shaker with a temperature of 20° C. and a rotation speed of 160 r / min for 30 hours to obtain a seed solution of flocculent bacteria. Other steps and parameters are the same as in the first embodiment.

specific Embodiment approach 3

[0016] Specific embodiment three: This embodiment is different from specific embodiment one in that in step one, it is placed in a shaker with a temperature of 30° C. and a rotating speed of 120 r / min for 18 hours to obtain a seed liquid of flocculent bacteria. Other steps and parameters are the same as in the first embodiment.

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Abstract

The invention relates to a bioflocculant fermentation method, in particular to a bioflocculant fermentation method with a mycelium pellet as a vector. The method solves the problems of high energy consumption, high production cost and unsuitability for large-scale industrialized fermentation production and the like existing in the existing bioflocculant fermentation method and comprises the following steps of: 1, mixing rhizobium radiobacter with bacillus sphaericus and then culturing so as to obtain flocculant-producing bacteria seed liquid; 2, culturing aspergillus niger to be a mature mycelium pellet; 3, mixing the mature mycelium pellet with the flocculant-producing bacteria seed liquid and then culturing so as to obtain a mixed mycelium pellet; 4, taking 24-hour as a fermentation period, and drawing out the mixed mycelium pellet after each fermentation period is finished and pouring the mixed mycelium pellet into fresh culture mediums for culturing; and 5, repetitively operating the step 4 for 30-35 times and namely completing. According to the bioflocculant fermentation method provided by the invention, on the premise of guaranteeing stable flocculating rate, seed liquid demand is reduced, production efficiency is increased and production energy consumption is reduced; moreover, the invention has great significance on large-scale industrialized production of bioflocculant.

Description

Technical field [0001] The invention relates to a method for fermenting a biological flocculant. Background technique [0002] Bioflocculant (BF) is a kind of natural biopolymer produced by microorganisms, which has flocculation and sedimentation functions for bacteria, cells, solid particles, colloidal particles and other suspended matter in liquid. Compared with chemical flocculants, biological flocculants have many advantages, such as high efficiency, non-toxicity, low fermentation cost, no secondary pollution, biodegradability, harmless degradation products, easy solid-liquid separation of products, etc., new environmentally friendly biological flocculation It meets the requirements of today's sustainable development and has important practical significance for human health and environmental protection. It has become one of the important directions of flocculant research and development, and it is a new type of green water purifying agent. [0003] The current research on biol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12R1/685C12R1/07C12R1/41
Inventor 马放王金娜李昂杨基先王继华邢洁张斯张淑娟
Owner HARBIN INST OF TECH
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