Bioflocculant fermentation method with mycelium pellet as vector
A technology of fermented organisms and mycelium balls, which is applied in the field of fermented biological flocculants, can solve the problems of being unsuitable for large-scale industrial fermentation production, low production efficiency, and high production costs, so as to reduce the chance of bacterial contamination, improve production efficiency, and flocculate The effect of rate stabilization
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specific Embodiment approach 1
[0009] Specific embodiment 1: The method of fermenting biological flocculant with mycelium pellets in this embodiment is carried out in the following steps: 1. Rhizobium radiata and Bacillus sphaericus are mixed in a volume ratio of 1:1 and then inoculated into 100 mL of flocculant. Culture culture medium in a 250mL Erlenmeyer flask, placed in a shaker with a temperature of 20~30℃ and a rotating speed of 120~160r / min for 18~30 hours to obtain a seed solution of flocculent fungus; 2. The spore suspension of Aspergillus niger Inoculate into a 250 mL Erlenmeyer flask containing 100 mL of mycelial pellet culture medium, and place it in a shaker at a temperature of 28-32°C and a rotation speed of 140r / min for 1-7 days to obtain mature hyphae; The mycelium pellets were rinsed with sterilized phosphate solution and added to a 250 mL Erlenmeyer flask containing 100 mL of flocculent culture medium, and then added the flocculent seed liquid at a volume ratio of 1% to 10%, and placed at a ...
specific Embodiment approach 2
[0015] Specific embodiment two: this embodiment is different from specific embodiment one in that in step one, it is placed in a shaker with a temperature of 20° C. and a rotation speed of 160 r / min for 30 hours to obtain a seed solution of flocculent bacteria. Other steps and parameters are the same as in the first embodiment.
specific Embodiment approach 3
[0016] Specific embodiment three: This embodiment is different from specific embodiment one in that in step one, it is placed in a shaker with a temperature of 30° C. and a rotating speed of 120 r / min for 18 hours to obtain a seed liquid of flocculent bacteria. Other steps and parameters are the same as in the first embodiment.
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