A kind of glycopeptide enrichment separation material and its application

Abstract

The invention discloses a glycopeptide enrichment and separation material and application thereof. The glycopeptide enrichment and separation material is a mixture of graphite carbon and active carbon in the ratio of 1:1. As proved by an experiment, the glycopeptide enrichment and separation material provided by the invention has a good enrichment effect on glycopeptide, the glycopeptide purity of an enrichment product is higher than 90 percent, and the recovery rate is higher than 85 percent. Compared with a mixture of graphite carbon and active carbon in the other ratios, the glycopeptide enrichment and separation material has the advantages that: the specificity of the material represented on glycopeptide is not resulted from easy addition action of the physical and chemical characteristics of the graphite carbon and active carbon but from the highly synergistic action of the graphite carbon and active carbon.

Description

technical field [0001] The invention relates to a glycopeptide enrichment and separation material and its application. Background technique [0002] Glycopeptides are peptides with covalently bonded sugar chains on the peptide chains, and are the products of enzymatic cleavage of glycoproteins. Due to the huge difference in the physical and chemical properties of glycopeptides and non-glycopeptides, non-glycopeptides have a serious inhibitory effect on glycopeptides when mass spectrometry is used to identify glycopeptides, making it difficult to detect and identify glycopeptides using conventional protein identification methods. The change of the sugar chain structure in glycoprotein is closely related to the occurrence, development and prognosis of the disease. The change of the sugar chain structure will be an important marker for predicting the disease process. key step. [0003] The enrichment of glycoproteins can be realized by using the characteristic that lectins ca...

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Problems solved by technology

[0003] The enrichment of glycoproteins can be realized by using the characteristic that lectins can specifically bind sugar chains, but the disadvantage of this method is that each lectin is only for one type of sugar chains, and it is still necessary to study the composition and structure of sugar chains. Peptides are enriched, and this method is expensive; based on the characteristics that the molecular weight of glycopeptides is much larger than that of non-glycopeptides, size exclusion chromatography can be used to enrich and separate glycopeptides, but the separation efficiency of this technology is low; One of the highly specific methods for glycopeptides. The disadvantage of this method is that the composition of sugar chains may be destroyed during the process of glycopeptide enrichment, and the obtained sugar chain information is incomplete; hydrophilic chromatography can enrich hydrophilic Glycopeptides can also enrich hydrophilic non-glycopeptides, but the purity of glycopeptides obtained by this method is low; functional magnetic beads (including boric acid functionalized magnetic beads and large-pore silica gel, etc.) are also used to enrich Glycopeptide collection, this type of technical method requires some chemical reactions, which increases the complexity of the enrichment process, limits the wide application of this method, and cannot achieve high-throughput glycopeptide enrichment; hydrophilic interaction liquid phase Chromatography and ion chromatography can also be used to enrich and separate glycopeptides from glycoprotein digestion products, but there are obvious preferences, and a large number of inorganic salt ions are introduced in the process of enriching glycopeptides, which increases the difficulty of subsequent sugar chain identification

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