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Hydrogen-oxidizing bacteria WMQ-7, and separation method and application thereof

A technology of hydrogen oxidation and bacteria, applied in the direction of biochemical equipment and methods, bacteria, methods based on microorganisms, etc., can solve problems that have not been popularized and applied in a large area

Inactive Publication Date: 2014-03-26
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to technical limitations, it has not been widely applied so far

Method used

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  • Hydrogen-oxidizing bacteria WMQ-7, and separation method and application thereof
  • Hydrogen-oxidizing bacteria WMQ-7, and separation method and application thereof
  • Hydrogen-oxidizing bacteria WMQ-7, and separation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Isolation of soil microorganism WMQ-7

[0074] 1. Sample collection: Collect vigorously growing HUP by conventional methods － Nodulated alfalfa rhizosphere (≤5 mm) soil.

[0075] 2. Soil enrichment: After the collected rhizosphere soil is sieved with a 16-mesh sieve, it is mixed with sterilized quartz sand (soil: quartz sand = 2:1) to prevent compaction. Put 60 mL of mixed soil into a glass tube (2.5 × 60 cm) with both ends closed, and then connect the three glass tubes in series. H is produced by electrolysis of water 2 , forming a flow rate of 280 mL / min, H 2 The content is 4.16×10 -4 -2.42×10 -3 mol / L mixed gas to enrich the hydrogen oxidizing bacteria in the soil. Similarly, 3 glass tubes connected in series were filled with 60 mL of mixed soil, and the group of soil samples was continuously ventilated with air as a control. Every 2 days, the gas chromatographic detection of the gas outlet V of the culture device 2 and V 4 H 2 content, make the hydrogen...

Embodiment 2

[0081] Example 2 Plant growth promoting effect of hydrogen oxidizing bacteria WMQ-7

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Abstract

The invention relates to Pseudomonasputida WMQ-7, which has been collected in China Typical Model Cultivation Center (CCTCC) of Wuhan University on March 8, 2011 with the collection number of CCTCC M 2011060, and also relates to a method for separating and culturing the Pseudomonasputida WMQ-7 in hydrogen-absorbing enzyme-free soil of the rhizosphere of a leguminous plant. The method comprises the following steps of: enriching, separating and culturing the Pseudomonasputida WMQ-7 by using a culture device for continuously introducing H2 and a mineral salt culture medium, and screening strains with higher H2 oxidizing capacity by using gas chromatography. A hydrogen bacterium preparation is prepared from the optimal strains through fermented in a laboratory. The biological preparation can promote plant growth and improve yield, fertilizer efficiency and stress resistance of plants when used for agricultural production.

Description

technical field [0001] The present invention relates to a hydrogen oxidizing bacterium WMQ-7 and its separation method and application, in particular to a hydrogen oxidizing bacterium Pseudomonas putida ( Pseudomonas putida ) WMQ-7 and its microbial preparations. The hydrogen oxidizing bacteria WMQ-7 strain was deposited in the Chinese Center for Typical Cultures of Wuhan University on March 8, 2011, and the preservation number is CCTCC M 2011060. Background technique [0002] The benefits of crop rotation and intercropping of legumes are mostly considered to be the effect of residual nitrogen from the roots of legumes, but recent studies have shown that nitrogen fertilizers cannot completely replace the effect of crop rotation of legumes, and about 75% of the yield-increasing effect cannot be used There are theories to explain. Studies have found that without hydrogen-absorbing enzyme (Without Uptake Hydrogenase, HUP － ) of rhizobia released H during nitrogen fixation 2 ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N11/04C12N11/14C05F11/08C12R1/40
Inventor 王卫卫蒙渊付博其他发明人请求不公开姓名
Owner NORTHWEST UNIV