Method for preparing sterile rose vine for tissue culture

Abstract

The invention relates to a rose tissue culture technique and aims to provide a method for preparing a sterile rose vine for tissue culture. The method comprises: trimming and washing a rose vine, soaking the rose vine in phenylthiocarbamide (PTC) aqueous solution at a concentration of 25 percent (v / v), and washing with distilled water; filling the rose vine forward and vertically into a tissue culture bottle which is sterilized by high-pressure steam, covering the bottle cap, placing the bottle in a 37 DEG C constant-temperature tank for 24 hours, transferring the bottle to a 4 DEG C refrigerator for 48 hours and repeating the step once; and cutting the rose vine into small sections with axillary buds under a sterile condition, sterilizing by soaking and in 70-percent alcohol and by shaking, taking the small sections out, washing the small sections with sterile water, soaking in 0.002 liter of mercury for 9 minutes, taking the small sections out, washing with sterile water, and obtaining the sterile rose vine for tissue culture. With the PTC aqueous solution and alternating treatment at 37 DEG C and 4 DEG C, the method has the advantages of higher sterile effect, higher germination rate, simpler and more convenient operation, time and labor conservation, lower cost, higher production application performance and the like compared with the conventional method.

Description

technical field [0001] The invention relates to rose tissue culture technology, in particular to a method for preparing sterile rose branches for tissue culture. Background technique [0002] Rose (Rosa rugosa) is a plant belonging to Rosaceae and the genus Rosa. Its flowers are beautiful in shape, fresh in color, and rich in fragrance. It has many uses such as food, medicine, health care, beauty, and ornamental. one of the flowers. Roses are also dominant tree species for soil and water conservation and urban garden construction. At present, roses are still propagated by traditional methods such as cuttings or grafting. There have long been a series of urgent problems that need to be solved, such as low reproductive coefficient, difficulty in breeding new varieties, serious bacterial infection, and difficulty in improving yield and quality. The use of tissue culture technology can realize the rapid breeding of fine varieties of roses, the selection of new varieties combin...

AI Technical Summary

Problems solved by technology

However, because there are often more fungi and bacteria in the rose body, and it is difficult to completely remove and obtain the aseptic explants required for tissue culture by general detoxification methods, the tissue culture cannot continue and eventually lead to failure