Chinese herbal medicine compound preparation for preventing and controlling fish saprolegnia and its application
A technology for compound preparations and Chinese herbal medicines, which can be used in pharmaceutical formulations, medical preparations containing active ingredients, antifungal agents, etc.
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[0012] Acute toxicity test
[0013] Take 150g of the preparation and soak it in distilled water for 4 hours, boil it with high heat, fry it with medium heat for 20 minutes, filter the liquid medicine, and then fry the second dose. The two doses are combined and filtered through a sterilized 200-mesh sieve to remove the medicine residue, and then use a triangular cone The bottle is concentrated into 100ml to make a liquid medicine with a concentration of 1.5g / ml. Store in a sterilized container at 4°C for later use.
[0014] The test fish were healthy, disease-free koi carp with a body weight of 75±5g, 10 fish in each group, a total of 260 fish. The test fish was temporarily raised for 7 days, and no death occurred. Inject 81L of water into a 120L aquarium, aerate for 48 hours, and conduct the test at a constant temperature of 20°C. Inject at the base of the dorsal fin, the injection depth is about 0.2cm. Stop eating, breathing and water within 4 days after injection. Obse...
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