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A kind of technology and application of extracting active protein from seaside mallow seeds

A protein, mallow seed technology, applied in peptide/protein components, medical preparations containing active ingredients, applications, etc.

Inactive Publication Date: 2011-12-21
HIGH TECH RES INST NANJING UNIV LIANYUNGANG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Experiments have shown that the nutrients in seashore mallow seeds have high nutritional value, and can be used as raw materials for oil plants, food, feed, health products and other industrial uses; however, there are few studies based on exploring its main active components. In recent years, only There are a handful of papers that show seaside mallow seeds have immune-boosting, blood-sugar-lowering effects
However, no papers have been found on the active protein components in mallow seeds
There is no patent literature on the preparation of mallow bioactive protein components from mallow seeds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1. The process of extracting active protein from seashore mallow seeds, its steps are as follows:

[0021] 1. 60 g of dried mallow seeds were taken, ground into a powder sample, extracted with petroleum ether (100 mL×3) under reflux at 80°C, and the defatted seed powder was dried in the shade at room temperature to obtain 51.7 g of defatted sample.

[0022] Take the obtained defatted seed powder sample, add 1100ml of distilled water at a ratio of 1:20 (w:v), and then use an ultrasonic instrument to ultrasonically destroy the cell membrane to assist in protein extraction. Ultrasonic conditions: temperature 40°C, power 200W, time 1h, use after ultrasonic Gauze filtration, repeat above steps, collect filtrate 1700ml.

[0023] Take DEAE-cellulose and treat it as usual. Pack the treated DEAE cellulose into a 25×4cm column and cover it with water. After the column is packed, use 0.01mol / L PBS (pH6.5) to pass through the column at a steady flow rate until equili...

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PUM

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Abstract

The invention relates to a technique for extracting active proteins from seashore mallow seeds, which is characterized by comprising the following steps: pulverizing seashore mallow seeds, degreasing, treating with ultrasonic, and filtering; and passing through DEAE (diethylaminoethanol)-cellulose columns, washing to remove impurities, eluting respectively with 0.01mol PBS (phosphate buffer solution), 0.01mol PBS-0.1mol NaCl / L and 0.01mol PBS-0.25mol NaCl / L, collecting, dialyzing, and carrying out freeze-drying to obtain the product. The active proteins extracted by the technique provided by the invention can be used for preparing health food or medicines for enhancing humoral immunity and liver tumor resistance, and can be made into capsules, tablets, oral liquids, injections and other health products and medicines as required according to the biological activities.

Description

technical field [0001] The invention belongs to the technical field of biochemical separation, and in particular relates to a process for extracting active protein from seaside mallow seeds; the invention also relates to the application of the aforementioned active protein. Background technique [0002] Seashore mallow (Kostelezkya virginica) is a perennial halophyte of the Malvaceae family, which is suitable for growing in coastal areas. It is naturally distributed in the salt marsh coastal zone of the eastern coast of the United States. It was introduced to China by Nanjing University in 1992. It has been reported in the literature that people pay more and more attention to salt-tolerant oil crops because of their resistance to salt water irrigation and high seed yield. Experiments have shown that the nutrients in seashore mallow seeds have high nutritional value, and can be used as raw materials for oil plants, food, feed, health products and other industrial uses; howev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/88C12N9/04C12N9/00A23L1/305A61K38/43A61K38/44A61K38/51A61P37/04A61P35/00A61P1/16
Inventor 钦佩张鹤云张太平
Owner HIGH TECH RES INST NANJING UNIV LIANYUNGANG
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